1q5y
From Proteopedia
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| - | [[Image:1q5y.jpg|left|200px]] | + | [[Image:1q5y.jpg|left|200px]] |
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| - | '''Nickel-Bound C-terminal Regulatory Domain of NikR''' | + | {{Structure |
| + | |PDB= 1q5y |SIZE=350|CAPTION= <scene name='initialview01'>1q5y</scene>, resolution 1.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene> and <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= NIKR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Nickel-Bound C-terminal Regulatory Domain of NikR''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Q5Y is a [ | + | 1Q5Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q5Y OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the nickel-responsive transcription factor NikR., Schreiter ER, Sintchak MD, Guo Y, Chivers PT, Sauer RT, Drennan CL, Nat Struct Biol. 2003 Oct;10(10):794-9. Epub 2003 Sep 14. PMID:[http:// | + | Crystal structure of the nickel-responsive transcription factor NikR., Schreiter ER, Sintchak MD, Guo Y, Chivers PT, Sauer RT, Drennan CL, Nat Struct Biol. 2003 Oct;10(10):794-9. Epub 2003 Sep 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12970756 12970756] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: regulatory domain]] | [[Category: regulatory domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:33:30 2008'' |
Revision as of 11:33, 20 March 2008
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| , resolution 1.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | NIKR (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Nickel-Bound C-terminal Regulatory Domain of NikR
Overview
NikR is a metal-responsive transcription factor that controls nickel uptake in Escherichia coli by regulating expression of a nickel-specific ATP-binding cassette (ABC) transporter. We have determined the first two structures of NikR: the full-length apo repressor at a resolution of 2.3 A and the nickel-bound C-terminal regulatory domain at a resolution of 1.4 A. NikR is the only known metal-responsive member of the ribbon-helix-helix family of transcription factors, and its structure has a quaternary arrangement consisting of two dimeric DNA-binding domains separated by a tetrameric regulatory domain that binds nickel. The position of the C-terminal regulatory domain enforces a large spacing between the contacts that each NikR DNA-binding domain can make with the nik operator. The regulatory domain of NikR contains four nickel-binding sites at the tetramer interface, each exhibiting a novel square-planar coordination by three histidines and one cysteine side chain.
About this Structure
1Q5Y is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of the nickel-responsive transcription factor NikR., Schreiter ER, Sintchak MD, Guo Y, Chivers PT, Sauer RT, Drennan CL, Nat Struct Biol. 2003 Oct;10(10):794-9. Epub 2003 Sep 14. PMID:12970756
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