1q8r
From Proteopedia
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| - | [[Image:1q8r.jpg|left|200px]] | + | [[Image:1q8r.jpg|left|200px]] |
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| - | '''Structure of E.coli RusA Holliday junction resolvase''' | + | {{Structure |
| + | |PDB= 1q8r |SIZE=350|CAPTION= <scene name='initialview01'>1q8r</scene>, resolution 1.899Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= rusa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of E.coli RusA Holliday junction resolvase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1Q8R is a [ | + | 1Q8R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q8R OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of Escherichia coli RusA endonuclease reveals a new Holliday junction DNA binding fold., Rafferty JB, Bolt EL, Muranova TA, Sedelnikova SE, Leonard P, Pasquo A, Baker PJ, Rice DW, Sharples GJ, Lloyd RG, Structure. 2003 Dec;11(12):1557-67. PMID:[http:// | + | The structure of Escherichia coli RusA endonuclease reveals a new Holliday junction DNA binding fold., Rafferty JB, Bolt EL, Muranova TA, Sedelnikova SE, Leonard P, Pasquo A, Baker PJ, Rice DW, Sharples GJ, Lloyd RG, Structure. 2003 Dec;11(12):1557-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14656440 14656440] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: recombination]] | [[Category: recombination]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:34:23 2008'' |
Revision as of 11:34, 20 March 2008
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| , resolution 1.899Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | rusa (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of E.coli RusA Holliday junction resolvase
Overview
Holliday junction resolution performed by a variety of structure-specific endonucleases is a key step in DNA recombination and repair. It is believed that all resolvases carry out their reaction chemistries in a similar fashion, utilizing a divalent cation to facilitate the hydrolysis of the phosphodiester backbone of the DNA, but their architecture varies. To date, with the exception of bacteriophage T4 endonuclease VII, each of the known resolvase enzyme structures has been categorized into one of two families: the integrases and the nucleases. We have now determined the structure of the Escherichia coli RusA Holliday junction resolvase, which reveals a fourth structural class for these enzymes. The structure suggests that dimer formation is essential for Mg(2+) cation binding and hence catalysis and that like the other resolvases, RusA distorts its Holliday junction target upon binding. Key residues identified by mutagenesis experiments are well positioned to interact with the DNA.
About this Structure
1Q8R is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The structure of Escherichia coli RusA endonuclease reveals a new Holliday junction DNA binding fold., Rafferty JB, Bolt EL, Muranova TA, Sedelnikova SE, Leonard P, Pasquo A, Baker PJ, Rice DW, Sharples GJ, Lloyd RG, Structure. 2003 Dec;11(12):1557-67. PMID:14656440
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