3kz3

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{{STRUCTURE_3kz3| PDB=3kz3 | SCENE= }}
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==A structure of a lambda repressor fragment mutant==
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===A structure of a lambda repressor fragment mutant===
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<StructureSection load='3kz3' size='340' side='right' caption='[[3kz3]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20138892}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3kz3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KZ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KZ3 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1lmb|1lmb]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kz3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kz3 RCSB], [http://www.ebi.ac.uk/pdbsum/3kz3 PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kz/3kz3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We survey the two-state to downhill folding transition by examining 20 lambda(6-85)* mutants that cover a wide range of stabilities and folding rates. We investigated four new lambda(6-85)* mutants designed to fold especially rapidly. Two were engineered using the core remodeling of Lim and Sauer, and two were engineered using Ferreiro et al.'s frustratometer. These proteins have probe-dependent melting temperatures as high as 80 degrees C and exhibit a fast molecular phase with the characteristic temperature dependence of the amplitude expected for downhill folding. The survey reveals a correlation between melting temperature and downhill folding previously observed for the beta-sheet protein WW domain. A simple model explains this correlation and predicts the melting temperature at which downhill folding becomes possible. An X-ray crystal structure with a 1.64-A resolution of a fast-folding mutant fragment shows regions of enhanced rigidity compared to the full wild-type protein.
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==Function==
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A survey of lambda repressor fragments from two-state to downhill folding.,Liu F, Gao YG, Gruebele M J Mol Biol. 2010 Apr 2;397(3):789-98. Epub 2010 Feb 6. PMID:20138892<ref>PMID:20138892</ref>
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[[http://www.uniprot.org/uniprot/RPC1_LAMBD RPC1_LAMBD]] Repressor protein CI allows phage lambda to reside inactively in the chromosome of its host bacterium. This lysogenic state is maintained by binding of regulatory protein CI to the OR and OL operators, preventing transcription of proteins necessary for lytic development.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3kz3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KZ3 OCA].
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</div>
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==Reference==
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==See Also==
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<ref group="xtra">PMID:020138892</ref><references group="xtra"/><references/>
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*[[Bacteriophage repressor|Bacteriophage repressor]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Enterobacteria phage lambda]]
[[Category: Enterobacteria phage lambda]]
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[[Category: Gao, Y.]]
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[[Category: Gao, Y]]
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[[Category: Gruebele, M.]]
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[[Category: Gruebele, M]]
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[[Category: Liu, F.]]
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[[Category: Liu, F]]
[[Category: Dna-binding]]
[[Category: Dna-binding]]
[[Category: Five helix bundle]]
[[Category: Five helix bundle]]

Revision as of 15:15, 18 December 2014

A structure of a lambda repressor fragment mutant

3kz3, resolution 1.64Å

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