1q9g

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[[Image:1q9g.jpg|left|200px]]<br /><applet load="1q9g" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1q9g.jpg|left|200px]]
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caption="1q9g" />
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'''NMR STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES'''<br />
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{{Structure
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|PDB= 1q9g |SIZE=350|CAPTION= <scene name='initialview01'>1q9g</scene>
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE= OMPX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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}}
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'''NMR STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1Q9G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q9G OCA].
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1Q9G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q9G OCA].
==Reference==
==Reference==
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NMR structure of the integral membrane protein OmpX., Fernandez C, Hilty C, Wider G, Guntert P, Wuthrich K, J Mol Biol. 2004 Mar 5;336(5):1211-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15037080 15037080]
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NMR structure of the integral membrane protein OmpX., Fernandez C, Hilty C, Wider G, Guntert P, Wuthrich K, J Mol Biol. 2004 Mar 5;336(5):1211-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15037080 15037080]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Wider, G.]]
[[Category: Wider, G.]]
[[Category: Wuthrich, K.]]
[[Category: Wuthrich, K.]]
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[[Category: detergents]]
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[[Category: detergent]]
[[Category: dhpc]]
[[Category: dhpc]]
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[[Category: lipids]]
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[[Category: lipid]]
[[Category: membrane protein]]
[[Category: membrane protein]]
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[[Category: micelles]]
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[[Category: micelle]]
[[Category: nmr]]
[[Category: nmr]]
[[Category: ompx]]
[[Category: ompx]]
[[Category: trosy]]
[[Category: trosy]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:37:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:34:43 2008''

Revision as of 11:34, 20 March 2008

Image:1q9g.jpg


PDB ID 1q9g

Drag the structure with the mouse to rotate
Gene: OMPX (Escherichia coli)
Coordinates: save as pdb, mmCIF, xml



NMR STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX IN DHPC MICELLES


Overview

The structure of the integral membrane protein OmpX from Escherichia coli reconstituted in 60 kDa DHPC micelles (OmpX/DHPC) was calculated from 526 NOE upper limit distance constraints. The structure determination was based on complete sequence-specific assignments for the amide protons and the Val, Leu, and Ile(delta1) methyl groups in OmpX, which were selectively protonated on a perdeuterated background. The solution structure of OmpX in the DHPC micelles consists of a well-defined, eight-stranded antiparallel beta-barrel, with successive pairs of beta-strands connected by mobile loops. Several long-range NOEs observed outside of the transmembrane barrel characterize an extension of a four-stranded beta-sheet beyond the height of the barrel. This protruding beta-sheet is believed to be involved in intermolecular interactions responsible for the biological functions of OmpX. The present approach for de novo structure determination should be quite widely applicable to membrane proteins reconstituted in mixed micelles with overall molecular masses up to about 100 kDa, and may also provide a platform for additional functional studies.

About this Structure

1Q9G is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

NMR structure of the integral membrane protein OmpX., Fernandez C, Hilty C, Wider G, Guntert P, Wuthrich K, J Mol Biol. 2004 Mar 5;336(5):1211-21. PMID:15037080

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