3ni0
From Proteopedia
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| - | + | ==Crystal Structure of Mouse BST-2/Tetherin Ectodomain== | |
| - | + | <StructureSection load='3ni0' size='340' side='right' caption='[[3ni0]], [[Resolution|resolution]] 1.60Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3ni0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NI0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NI0 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Bst2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ni0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ni0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ni0 RCSB], [http://www.ebi.ac.uk/pdbsum/3ni0 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | BST-2/tetherin is a host anti-viral molecule that functions to potently inhibit the release of enveloped viruses from infected cells. In return, viruses have evolved antagonists to this activity. BST-2 traps budding virions by using two separate membrane-anchoring regions that simultaneously incorporate into the host and viral membrane. Here we detail the structural and biophysical properties of the full BST-2 ectodomain, which spans the two membrane anchors. The 1.6 Angstrom crystal structure of the complete mouse BST-2 ectodomain reveals a ~145 Angstrom parallel dimer in an extended alpha-helix conformation that predominantly forms a coiled-coil bridged by three intermolecular disulfides that are required for stability. Sequence analysis in the context of the structure reveals an evolutionarily conserved design that destabilizes the coiled-coil, resulting in a labile superstructure, as evidenced by solution X-ray scattering displaying bent conformations spanning 150 Angstrom and 180 Angstrom for mouse and human BST-2 ectodomains, respectively. Additionally, crystal packing analysis reveals possible curvature-sensing tetrameric structures that may aid in proper placement of BST-2 during the genesis of viral progeny. Overall, this extended coiled-coil structure with inherent plasticity is undoubtedly necessary to accommodate the dynamics of virus budding while ensuring separation of the anchors. | ||
| - | + | Structural and biophysical analysis of BST-2/tetherin ectodomains reveal an evolutionary conserved design to inhibit virus release.,Swiecki M, Scheaffer SM, Allaire M, Fremont DH, Colonna M, Brett TJ J Biol Chem. 2010 Nov 17. PMID:21084286<ref>PMID:21084286</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
| - | [[Category: Brett, T J | + | [[Category: Brett, T J]] |
| - | [[Category: Scheaffer, S M | + | [[Category: Scheaffer, S M]] |
[[Category: Antiviral defense]] | [[Category: Antiviral defense]] | ||
[[Category: Coiled-coil]] | [[Category: Coiled-coil]] | ||
[[Category: Gpi anchor]] | [[Category: Gpi anchor]] | ||
[[Category: Immune system]] | [[Category: Immune system]] | ||
Revision as of 15:24, 18 December 2014
Crystal Structure of Mouse BST-2/Tetherin Ectodomain
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