This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1qbk

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1qbk.gif|left|200px]]<br /><applet load="1qbk" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1qbk.gif|left|200px]]
-
caption="1qbk, resolution 3.0&Aring;" />
+
 
-
'''STRUCTURE OF THE KARYOPHERIN BETA2-RAN GPPNHP NUCLEAR TRANSPORT COMPLEX'''<br />
+
{{Structure
 +
|PDB= 1qbk |SIZE=350|CAPTION= <scene name='initialview01'>1qbk</scene>, resolution 3.0&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER'>GNP</scene>
 +
|ACTIVITY=
 +
|GENE=
 +
}}
 +
 
 +
'''STRUCTURE OF THE KARYOPHERIN BETA2-RAN GPPNHP NUCLEAR TRANSPORT COMPLEX'''
 +
 
==Overview==
==Overview==
Line 10: Line 19:
==About this Structure==
==About this Structure==
-
1QBK is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GNP:'>GNP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QBK OCA].
+
1QBK is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QBK OCA].
==Reference==
==Reference==
-
Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp., Chook YM, Blobel G, Nature. 1999 May 20;399(6733):230-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10353245 10353245]
+
Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp., Chook YM, Blobel G, Nature. 1999 May 20;399(6733):230-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10353245 10353245]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Protein complex]]
Line 20: Line 29:
[[Category: GNP]]
[[Category: GNP]]
[[Category: MG]]
[[Category: MG]]
-
[[Category: heat repeats]]
+
[[Category: heat repeat]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:37:58 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:35:28 2008''

Revision as of 11:35, 20 March 2008

Image:1qbk.gif


PDB ID 1qbk

Drag the structure with the mouse to rotate
, resolution 3.0Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF THE KARYOPHERIN BETA2-RAN GPPNHP NUCLEAR TRANSPORT COMPLEX


Contents

Overview

Transport factors in the karyopherin-beta (also called importin-beta) family mediate the movement of macromolecules in nuclear-cytoplasmic transport pathways. Karyopherin-beta2 (transportin) binds a cognate import substrate and targets it to the nuclear pore complex. In the nucleus, Ran x GTP binds karyopherin-beta2 and dissociates the substrate. Here we present the 3.0 A structure of the karyopherin-beta2-Ran x GppNHp complex where GppNHp is a non-hydrolysable GTP analogue. Karyopherin-beta2 contains eighteen HEAT repeats arranged into two continuous orthogonal arches. Ran is clamped in the amino-terminal arch and substrate-binding activity is mapped to the carboxy-terminal arch. A large loop in HEAT repeat 7 spans both arches. Interactions of the loop with Ran and the C-terminal arch implicate it in GTPase-mediated dissociation of the import-substrate. Ran x GppNHp in the complex shows extensive structural rearrangement, compared to Ran GDP, in regions contacting karyopherin-beta2. This provides a structural basis for the specificity of the karyopherin-beta family for the GTP-bound state of Ran, as well as a rationale for interactions of the karyopherin-Ran complex with the regulatory proteins ranGAP, ranGEF and ranBP1.

Disease

Known diseases associated with this structure: Deafness, mitochondrial, modifier of OMIM:[610230], Osteolysis, familial expansile OMIM:[603499], Paget disease of bone OMIM:[603499]

About this Structure

1QBK is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp., Chook YM, Blobel G, Nature. 1999 May 20;399(6733):230-7. PMID:10353245

Page seeded by OCA on Thu Mar 20 13:35:28 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qbk"
Personal tools