1qcj
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1qcj.gif|left|200px]] | + | [[Image:1qcj.gif|left|200px]] |
| - | + | ||
| - | '''LOW TEMPERATURE COMPLEX OF POKEWEED ANTIVIRAL PROTEIN WITH PTEORIC ACID''' | + | {{Structure |
| + | |PDB= 1qcj |SIZE=350|CAPTION= <scene name='initialview01'>1qcj</scene>, resolution 2.1Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=APT:2-AMINO-6-[(4-CARBOXY-PHENYLAMINO)-METHYL]-4-HYDROXY-PTERIDIN-1-IUM'>APT</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''LOW TEMPERATURE COMPLEX OF POKEWEED ANTIVIRAL PROTEIN WITH PTEORIC ACID''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1QCJ is a [ | + | 1QCJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Phytolacca_americana Phytolacca americana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QCJ OCA]. |
==Reference== | ==Reference== | ||
| - | X-ray crystallographic analysis of the structural basis for the interactions of pokeweed antiviral protein with its active site inhibitor and ribosomal RNA substrate analogs., Kurinov IV, Myers DE, Irvin JD, Uckun FM, Protein Sci. 1999 Sep;8(9):1765-72. PMID:[http:// | + | X-ray crystallographic analysis of the structural basis for the interactions of pokeweed antiviral protein with its active site inhibitor and ribosomal RNA substrate analogs., Kurinov IV, Myers DE, Irvin JD, Uckun FM, Protein Sci. 1999 Sep;8(9):1765-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10493577 10493577] |
[[Category: Phytolacca americana]] | [[Category: Phytolacca americana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 21: | Line 30: | ||
[[Category: pteoric acid]] | [[Category: pteoric acid]] | ||
[[Category: ribosome inactivating protein]] | [[Category: ribosome inactivating protein]] | ||
| - | [[Category: rna substrate | + | [[Category: rna substrate analog]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:35:49 2008'' |
Revision as of 11:35, 20 March 2008
| |||||||
| , resolution 2.1Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
LOW TEMPERATURE COMPLEX OF POKEWEED ANTIVIRAL PROTEIN WITH PTEORIC ACID
Overview
The pokeweed antiviral protein (PAP) belongs to a family of ribosome-inactivating proteins (RIP), which depurinate ribosomal RNA through their site-specific N-glycosidase activity. We report low temperature, three-dimensional structures of PAP co-crystallized with adenyl-guanosine (ApG) and adenyl-cytosine-cytosine (ApCpC). Crystal structures of 2.0-2.1 A resolution revealed that both ApG or ApCpC nucleotides are cleaved by PAP, leaving only the adenine base clearly visible in the active site pocket of PAP. ApCpC does not resemble any known natural substrate for any ribosome-inactivating proteins and its cleavage by PAP provides unprecedented evidence for a broad spectrum N-glycosidase activity of PAP toward adenine-containing single stranded RNA. We also report the analysis of a 2.1 A crystal structure of PAP complexed with the RIP inhibitor pteoric acid. The pterin ring is strongly bound in the active site, forming four hydrogen bonds with active site residues and one hydrogen bond with the coordinated water molecule. The second 180 degrees rotation conformation of pterin ring can form only three hydrogen bonds in the active site and is less energetically favorable. The benzoate moiety is parallel to the protein surface of PAP and forms only one hydrogen bond with the guanido group of Arg135.
About this Structure
1QCJ is a Single protein structure of sequence from Phytolacca americana. Full crystallographic information is available from OCA.
Reference
X-ray crystallographic analysis of the structural basis for the interactions of pokeweed antiviral protein with its active site inhibitor and ribosomal RNA substrate analogs., Kurinov IV, Myers DE, Irvin JD, Uckun FM, Protein Sci. 1999 Sep;8(9):1765-72. PMID:10493577
Page seeded by OCA on Thu Mar 20 13:35:49 2008
