3n0f

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{{STRUCTURE_3n0f| PDB=3n0f | SCENE= }}
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==Crystal Structure of Isoprene Synthase from Grey Poplar Leaves (Populus x canescens)==
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===Crystal Structure of Isoprene Synthase from Grey Poplar Leaves (Populus x canescens)===
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<StructureSection load='3n0f' size='340' side='right' caption='[[3n0f]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20624401}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3n0f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Populus_tremula_x_populus_alba Populus tremula x populus alba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N0F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3N0F FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3n0g|3n0g]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ispS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=80863 Populus tremula x Populus alba])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Isoprene_synthase Isoprene synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.27 4.2.3.27] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3n0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n0f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3n0f RCSB], [http://www.ebi.ac.uk/pdbsum/3n0f PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n0/3n0f_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The X-ray crystal structure of recombinant PcISPS (isoprene synthase from gray poplar hybrid Populusxcanescens) has been determined at 2.7 A resolution, and the structure of its complex with three Mg(2+) and the unreactive substrate analogue dimethylallyl-S-thiolodiphosphate has been determined at 2.8 A resolution. Analysis of these structures suggests that the generation of isoprene from substrate dimethylallyl diphosphate occurs via a syn-periplanar elimination mechanism in which the diphosphate-leaving group serves as a general base. This chemical mechanism is responsible for the annual atmospheric emission of 100 Tg of isoprene by terrestrial plant life. Importantly, the PcISPS structure promises to guide future protein engineering studies, potentially leading to hydrocarbon fuels and products that do not rely on traditional petrochemical sources.
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==About this Structure==
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Structure of Isoprene Synthase Illuminates the Chemical Mechanism of Teragram Atmospheric Carbon Emission.,Koksal M, Zimmer I, Schnitzler JP, Christianson DW J Mol Biol. 2010 Jul 17. PMID:20624401<ref>PMID:20624401</ref>
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[[3n0f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Populus_tremula_x_populus_alba Populus tremula x populus alba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N0F OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:020624401</ref><references group="xtra"/><references/>
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</div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Isoprene synthase]]
[[Category: Isoprene synthase]]
[[Category: Populus tremula x populus alba]]
[[Category: Populus tremula x populus alba]]
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[[Category: Christianson, D W.]]
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[[Category: Christianson, D W]]
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[[Category: Koksal, M.]]
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[[Category: Koksal, M]]
[[Category: Ddxxd motif]]
[[Category: Ddxxd motif]]
[[Category: Hemiterpene synthase]]
[[Category: Hemiterpene synthase]]

Revision as of 15:54, 18 December 2014

Crystal Structure of Isoprene Synthase from Grey Poplar Leaves (Populus x canescens)

3n0f, resolution 2.70Å

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