3jyo
From Proteopedia
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| - | + | ==Quinate dehydrogenase from Corynebacterium glutamicum in complex with NAD== | |
| - | ===Quinate dehydrogenase from Corynebacterium glutamicum in complex with NAD | + | <StructureSection load='3jyo' size='340' side='right' caption='[[3jyo]], [[Resolution|resolution]] 1.00Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3jyo]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Corynebacterium_glutamicum_atcc_13032 Corynebacterium glutamicum atcc 13032]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JYO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JYO FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2nlo|2nlo]], [[3jyp|3jyp]], [[3jyq|3jyq]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aroE, cg0504, Cgl0424 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196627 Corynebacterium glutamicum ATCC 13032])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jyo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3jyo RCSB], [http://www.ebi.ac.uk/pdbsum/3jyo PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Abstract Quinate dehydrogenase (QDH) catalyzes the reversible oxidation of quinate to 3-dehydroquinate by NADH and is involved in the catabolic quinate metabolism required for the degradation of lignin. The enzyme is a member of the family of shikimate/quinate dehydrogenases (SDH/QDH) occurring in bacteria and plants. We characterized the dual-substrate quinate/shikimate dehydrogenase QSDH from Corynebacterium glutamicum (CglQSDH) kinetically and revealed a clear substrate preference of CglQSDH for quinate compared to shikimate both at the pH-optimum and in a physiological pH-range, which is a remarkable contrast to closely related SDH/QDH enzymes. With respect to the cosubstrate CglQSDH is strictly NAD(H) dependent. These substrate and cosubstrate profiles correlate nicely to the details of three atomic resolution crystal structures of CglQSDH in different functional states we report here: with bound NAD+ (binary complex) and as ternary complexes with NADH plus either shikimate or quinate. The CglQSDH-NADH-quinate structure is the first complex structure of any member of the SDH/QDH family with quinate. Based on this novel structural information and systematic sequence and structure comparisons with closely related enzymes we can explain the strict NAD(H) dependency of CglQSDH as well as its discrimination between shikimate and quinate. | ||
| - | + | Enzyme-substrate complexes of the quinate/shikimate dehydrogenase from Corynebacterium glutamicum enable new insights in substrate and cofactor binding, specificity and discrimination.,Hoppner A, Schomburg D, Niefind K Biol Chem. 2013 Aug 8. pii:, /j/bchm.just-accepted/hsz-2013-0170/hsz-2013-0170.xml. doi:, 10.1515/hsz-2013-0170. PMID:23929881<ref>PMID:23929881</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | == | + | ==See Also== |
| - | + | *[[Shikimate dehydrogenase|Shikimate dehydrogenase]] | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Corynebacterium glutamicum atcc 13032]] | [[Category: Corynebacterium glutamicum atcc 13032]] | ||
| - | [[Category: Hoeppner, A | + | [[Category: Hoeppner, A]] |
| - | [[Category: Niefind, K | + | [[Category: Niefind, K]] |
| - | [[Category: Schomburg, D | + | [[Category: Schomburg, D]] |
[[Category: Amino-acid biosynthesis]] | [[Category: Amino-acid biosynthesis]] | ||
[[Category: Aromatic amino acid biosynthesis]] | [[Category: Aromatic amino acid biosynthesis]] | ||
Revision as of 15:54, 18 December 2014
Quinate dehydrogenase from Corynebacterium glutamicum in complex with NAD
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