3ngg
From Proteopedia
(Difference between revisions)
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| - | + | ==X-ray Structure of Omwaprin== | |
| - | === | + | <StructureSection load='3ngg' size='340' side='right' caption='[[3ngg]], [[Resolution|resolution]] 1.33Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3ngg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NGG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NGG FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ngg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ngg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ngg RCSB], [http://www.ebi.ac.uk/pdbsum/3ngg PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ng/3ngg_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The 50-residue snake venom protein L-omwaprin and its enantiomer D-omwaprin were prepared by total chemical synthesis. Radial diffusion assays were performed against Bacillus megaterium and Bacillus anthracis; both L-omwaprin and D-omwaprin showed anti-bacterial activity against B. megaterium. The native protein enantiomer, made of L- amino acids, failed to crystallize readily. However, when a racemic mixture containing equal amounts of L-omwaprin and D-omwaprin was used, diffraction quality crystals were obtained. The racemic protein sample crystallized in the centrosymmetric space group P2(1)/c and its structure was determined at atomic resolution (1.33 A) by a combination of Patterson and direct methods based on the strong scattering from the sulfur atoms in the eight cysteine residues per protein. Racemic crystallography once again proved to be a valuable method for obtaining crystals of recalcitrant proteins, and for determining high resolution X-ray structures by direct methods. | ||
| - | + | Determination of the X-ray structure of the snake venom protein omwaprin by total chemical synthesis and racemic protein crystallography.,Banigan JR, Mandal K, Sawaya MR, Thammavongsa V, Hendrickx AP, Schneewind O, Yeates TO, H Kent SB Protein Sci. 2010 Jul 28. PMID:20669184<ref>PMID:20669184</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| - | [[Category: Banigan, J R | + | == References == |
| - | [[Category: Hendrickx, A P.A | + | <references/> |
| - | [[Category: Kent, S B.H | + | __TOC__ |
| - | [[Category: Mandal, K | + | </StructureSection> |
| - | [[Category: Sawaya, M R | + | [[Category: Banigan, J R]] |
| - | [[Category: Schneewind, O | + | [[Category: Hendrickx, A P.A]] |
| - | [[Category: Thammavongsa, V | + | [[Category: Kent, S B.H]] |
| - | [[Category: Yeates, T O | + | [[Category: Mandal, K]] |
| + | [[Category: Sawaya, M R]] | ||
| + | [[Category: Schneewind, O]] | ||
| + | [[Category: Thammavongsa, V]] | ||
| + | [[Category: Yeates, T O]] | ||
[[Category: Antibiotic]] | [[Category: Antibiotic]] | ||
[[Category: Direct method]] | [[Category: Direct method]] | ||
[[Category: Racemic protein crystallography]] | [[Category: Racemic protein crystallography]] | ||
[[Category: Venom protein]] | [[Category: Venom protein]] | ||
Revision as of 15:59, 18 December 2014
X-ray Structure of Omwaprin
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