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3mq1
From Proteopedia
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| - | + | ==Crystal Structure of Dust Mite Allergen Der p 5== | |
| - | + | <StructureSection load='3mq1' size='340' side='right' caption='[[3mq1]], [[Resolution|resolution]] 2.80Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3mq1]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Dermatophagoides_pteronyssinus Dermatophagoides pteronyssinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MQ1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MQ1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DERP5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6956 Dermatophagoides pteronyssinus])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mq1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mq1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mq1 RCSB], [http://www.ebi.ac.uk/pdbsum/3mq1 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mq/3mq1_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Group 5 allergens from house dust mites elicit strong IgE antibody binding in mite-allergic patients. The structure of Der p 5 was determined by x-ray crystallography to better understand the IgE epitopes, to investigate the biologic function in mites, and to compare with the conflicting published Blo t 5 structures, designated 2JMH and 2JRK in the Protein Data Bank. Der p 5 is a three-helical bundle similar to Blo t 5, but the interactions of the helices are more similar to 2JMH than 2JRK. The crystallographic asymmetric unit contains three dimers of Der p 5 that are not exactly alike. Solution scattering techniques were used to assess the multimeric state of Der p 5 in vitro and showed that the predominant state was monomeric, similar to Blo t 5, but larger multimeric species are also present. In the crystal, the formation of the Der p 5 dimer creates a large hydrophobic cavity of approximately 3000 A(3) that could be a ligand-binding site. Many allergens are known to bind hydrophobic ligands, which are thought to stimulate the innate immune system and have adjuvant-like effects on IgE-mediated inflammatory responses. | ||
| - | + | Der p 5 crystal structure provides insight into the group 5 dust mite allergens.,Mueller GA, Gosavi RA, Krahn JM, Edwards LL, Cuneo MJ, Glesner J, Pomes A, Chapman MD, London RE, Pedersen LC J Biol Chem. 2010 Aug 13;285(33):25394-401. Epub 2010 Jun 9. PMID:20534590<ref>PMID:20534590</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Dermatophagoides pteronyssinus]] | [[Category: Dermatophagoides pteronyssinus]] | ||
| - | [[Category: Chapman, M D | + | [[Category: Chapman, M D]] |
| - | [[Category: Cuneo, M J | + | [[Category: Cuneo, M J]] |
| - | [[Category: Edwards, L L | + | [[Category: Edwards, L L]] |
| - | [[Category: Glesner, J | + | [[Category: Glesner, J]] |
| - | [[Category: Gosavi, R A | + | [[Category: Gosavi, R A]] |
| - | [[Category: Krahn, J M | + | [[Category: Krahn, J M]] |
| - | [[Category: London, R E | + | [[Category: London, R E]] |
| - | [[Category: Mueller, G A | + | [[Category: Mueller, G A]] |
| - | [[Category: Pedersen, L C | + | [[Category: Pedersen, L C]] |
| - | [[Category: Pomes, A | + | [[Category: Pomes, A]] |
[[Category: Allergen]] | [[Category: Allergen]] | ||
[[Category: Dust mite]] | [[Category: Dust mite]] | ||
Revision as of 16:03, 18 December 2014
Crystal Structure of Dust Mite Allergen Der p 5
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