1qg1

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[[Image:1qg1.gif|left|200px]]<br /><applet load="1qg1" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1qg1.gif|left|200px]]
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caption="1qg1" />
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'''GROWTH FACTOR RECEPTOR BINDING PROTEIN SH2 DOMAIN COMPLEXED WITH AN SHC-DERIVED PEPTIDE'''<br />
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{{Structure
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|PDB= 1qg1 |SIZE=350|CAPTION= <scene name='initialview01'>1qg1</scene>
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|SITE=
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|LIGAND=
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|ACTIVITY=
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}}
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'''GROWTH FACTOR RECEPTOR BINDING PROTEIN SH2 DOMAIN COMPLEXED WITH AN SHC-DERIVED PEPTIDE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1QG1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QG1 OCA].
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1QG1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QG1 OCA].
==Reference==
==Reference==
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Solution structure of the SH2 domain of Grb2 complexed with the Shc-derived phosphotyrosine-containing peptide., Ogura K, Tsuchiya S, Terasawa H, Yuzawa S, Hatanaka H, Mandiyan V, Schlessinger J, Inagaki F, J Mol Biol. 1999 Jun 11;289(3):439-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10356320 10356320]
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Solution structure of the SH2 domain of Grb2 complexed with the Shc-derived phosphotyrosine-containing peptide., Ogura K, Tsuchiya S, Terasawa H, Yuzawa S, Hatanaka H, Mandiyan V, Schlessinger J, Inagaki F, J Mol Biol. 1999 Jun 11;289(3):439-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10356320 10356320]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: signal transduction]]
[[Category: signal transduction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:39:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:36:54 2008''

Revision as of 11:36, 20 March 2008


PDB ID 1qg1

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GROWTH FACTOR RECEPTOR BINDING PROTEIN SH2 DOMAIN COMPLEXED WITH AN SHC-DERIVED PEPTIDE


Contents

Overview

The solution structure of growth factor receptor-bound protein 2 (Grb2) SH2 complexed with a Shc-derived phosphotyrosine (pTyr)-containing peptide was determined by nuclear magnetic resonance (NMR) spectroscopy. The pTyr binding site of Grb2 SH2 was similar to those of other SH2 domains. In contrast, the amino acid residues C-terminal to pTyr did not form an extended structure because of steric hindrance caused by a bulky side-chain of Trp121 (EF1). As a result, the peptide formed a turn-structure on the surface of Grb2 SH2. The asparagine residue at the pTyr+2 position of the Shc-peptide interacted with the main-chain carbonyl groups of Lys109 and Leu120. The present solution structure was similar to the crystal structure reported for Grb2 SH2 complexed with a BCR-Abl-derived phosphotyrosine-containing peptide. Finally, the structure of Grb2 SH2 domain was compared with those of the complexes of Src and phospholipase C-gamma1 with their cognate peptides, showing that the specific conformation of the peptide was required for binding to the SH2 domains.

Disease

Known diseases associated with this structure: Central hypoventilation syndrome, congenital OMIM:[100790], Haddad syndrome OMIM:[100790]

About this Structure

1QG1 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Solution structure of the SH2 domain of Grb2 complexed with the Shc-derived phosphotyrosine-containing peptide., Ogura K, Tsuchiya S, Terasawa H, Yuzawa S, Hatanaka H, Mandiyan V, Schlessinger J, Inagaki F, J Mol Biol. 1999 Jun 11;289(3):439-45. PMID:10356320

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