1qg7
From Proteopedia
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| - | [[Image:1qg7.jpg|left|200px]] | + | [[Image:1qg7.jpg|left|200px]] |
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| - | '''STROMA CELL-DERIVED FACTOR-1ALPHA (SDF-1ALPHA)''' | + | {{Structure |
| + | |PDB= 1qg7 |SIZE=350|CAPTION= <scene name='initialview01'>1qg7</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STROMA CELL-DERIVED FACTOR-1ALPHA (SDF-1ALPHA)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1QG7 is a [ | + | 1QG7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QG7 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of recombinant native SDF-1alpha with additional mutagenesis studies: an attempt at a more comprehensive interpretation of accumulated structure-activity relationship data., Ohnishi Y, Senda T, Nandhagopal N, Sugimoto K, Shioda T, Nagal Y, Mitsui Y, J Interferon Cytokine Res. 2000 Aug;20(8):691-700. PMID:[http:// | + | Crystal structure of recombinant native SDF-1alpha with additional mutagenesis studies: an attempt at a more comprehensive interpretation of accumulated structure-activity relationship data., Ohnishi Y, Senda T, Nandhagopal N, Sugimoto K, Shioda T, Nagal Y, Mitsui Y, J Interferon Cytokine Res. 2000 Aug;20(8):691-700. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10954912 10954912] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: cxc-chemokine]] | [[Category: cxc-chemokine]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:37:05 2008'' |
Revision as of 11:37, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
STROMA CELL-DERIVED FACTOR-1ALPHA (SDF-1ALPHA)
Contents |
Overview
Crystal structures, forms 1 and 2, of recombinant native stromal cell-derived factor-1alpha (SDF-1alpha), expressed using the Sendai virus expression vector system, have been determined by x-ray crystallography at 2.0 A resolution. The crystal of form 1 is almost isomorphous with that used in the previous crystal structure analysis of the synthetic [N33A] mutant of SDF-1alpha (Dealwis, C., et al. Proc. Natl. Acad. Sci. USA 1998;95, 6941-6946). However, the present structure analysis led to considerably better refinement statistics, revealing an error in the structural assignment of N-terminal residues in the previous report. Comparison of the solution structure, as previously determined by nuclear magnetic resonance (NMR) spectroscopy, and the present structure, with two monomers in the asymmetric unit, reveals several local conformational differences. Alanine scan mutagenesis studies for each residue in the so-called RFFESH motif revealed that only the first residue, Arg12, is effective in enhancing receptor binding (and successive activation). A new notion that steric restraint between Arg8 and Arg12 is favorable (if not vital) for retaining SDF activities appears to explain more consistently the structure-activity relationship data accumulated to date. Four guiding principles are presented that may be useful for designing potent therapeutic compounds interfering with HIV-1 infection through competition at the CXCR4 coreceptor.
Disease
Known diseases associated with this structure: AIDS, resistance to OMIM:[600835]
About this Structure
1QG7 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of recombinant native SDF-1alpha with additional mutagenesis studies: an attempt at a more comprehensive interpretation of accumulated structure-activity relationship data., Ohnishi Y, Senda T, Nandhagopal N, Sugimoto K, Shioda T, Nagal Y, Mitsui Y, J Interferon Cytokine Res. 2000 Aug;20(8):691-700. PMID:10954912
Page seeded by OCA on Thu Mar 20 13:37:05 2008
