1qgn
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1qgn.jpg|left|200px]] | + | [[Image:1qgn.jpg|left|200px]] |
| - | + | ||
| - | '''CYSTATHIONINE GAMMA-SYNTHASE FROM NICOTIANA TABACUM''' | + | {{Structure |
| + | |PDB= 1qgn |SIZE=350|CAPTION= <scene name='initialview01'>1qgn</scene>, resolution 2.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Cystathionine_gamma-synthase Cystathionine gamma-synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.48 2.5.1.48] | ||
| + | |GENE= METB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4097 Nicotiana tabacum]) | ||
| + | }} | ||
| + | |||
| + | '''CYSTATHIONINE GAMMA-SYNTHASE FROM NICOTIANA TABACUM''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1QGN is a [ | + | 1QGN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QGN OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of cystathionine gamma-synthase from Nicotiana tabacum reveals its substrate and reaction specificity., Steegborn C, Messerschmidt A, Laber B, Streber W, Huber R, Clausen T, J Mol Biol. 1999 Jul 30;290(5):983-96. PMID:[http:// | + | The crystal structure of cystathionine gamma-synthase from Nicotiana tabacum reveals its substrate and reaction specificity., Steegborn C, Messerschmidt A, Laber B, Streber W, Huber R, Clausen T, J Mol Biol. 1999 Jul 30;290(5):983-96. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10438597 10438597] |
[[Category: Cystathionine gamma-synthase]] | [[Category: Cystathionine gamma-synthase]] | ||
[[Category: Nicotiana tabacum]] | [[Category: Nicotiana tabacum]] | ||
| Line 25: | Line 34: | ||
[[Category: pyridoxal 5'-phosphate]] | [[Category: pyridoxal 5'-phosphate]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:37:16 2008'' |
Revision as of 11:37, 20 March 2008
| |||||||
| , resolution 2.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | METB (Nicotiana tabacum) | ||||||
| Activity: | Cystathionine gamma-synthase, with EC number 2.5.1.48 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CYSTATHIONINE GAMMA-SYNTHASE FROM NICOTIANA TABACUM
Overview
Cystathionine gamma-synthase catalyses the committed step of de novo methionine biosynthesis in micro-organisms and plants, making the enzyme an attractive target for the design of new antibiotics and herbicides. The crystal structure of cystathionine gamma-synthase from Nicotiana tabacum has been solved by Patterson search techniques using the structure of Escherichia coli cystathionine gamma-synthase. The model was refined at 2.9 A resolution to a crystallographic R -factor of 20.1 % (Rfree25.0 %). The physiological substrates of the enzyme, L-homoserine phosphate and L-cysteine, were modelled into the unliganded structure. These complexes support the proposed ping-pong mechanism for catalysis and illustrate the dissimilar substrate specificities of bacterial and plant cystathionine gamma-synthases on a molecular level. The main difference arises from the binding modes of the distal substrate groups (O -acetyl/succinyl versusO -phosphate). Central in fixing the distal phosphate of the plant CGS substrate is an exposed lysine residue that is strictly conserved in plant cystathionine gamma-synthases whereas bacterial enzymes carry a glycine residue at this position. General insight regarding the reaction specificity of transsulphuration enzymes is gained by the comparison to cystathionine beta-lyase from E. coli, indicating the mechanistic importance of a second substrate binding site for L-cysteine which leads to different chemical reaction types.
About this Structure
1QGN is a Single protein structure of sequence from Nicotiana tabacum. Full crystallographic information is available from OCA.
Reference
The crystal structure of cystathionine gamma-synthase from Nicotiana tabacum reveals its substrate and reaction specificity., Steegborn C, Messerschmidt A, Laber B, Streber W, Huber R, Clausen T, J Mol Biol. 1999 Jul 30;290(5):983-96. PMID:10438597
Page seeded by OCA on Thu Mar 20 13:37:16 2008
