3mha
From Proteopedia
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| - | + | ==Crystal structure of LprG from Mycobacterium tuberculosis bound to PIM== | |
| - | + | <StructureSection load='3mha' size='340' side='right' caption='[[3mha]], [[Resolution|resolution]] 1.85Å' scene=''> | |
| - | {{ | + | == Structural highlights == |
| + | <table><tr><td colspan='2'>[[3mha]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MHA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MHA FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=Z69:(1S,2R,3R,4S,5R,6S)-2-(ALPHA-L-ALLOPYRANOSYLOXY)-3,4,5-TRIHYDROXY-6-({6-O-[(1R)-1-HYDROXYHEXADECYL]-BETA-L-GULOPYRANOSYL}OXY)CYCLOHEXYL+(2R)-2-{[(1S)-1-HYDROXYHEXADECYL]OXY}-3-{[(1S,10S)-1-HYDROXY-10-METHYLOCTADECYL]OXY}PROPYL+HYDROGEN+(R)-PHOSPHATE'>Z69</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3mh8|3mh8]], [[3mh9|3mh9]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lpp-27, lprG, MT1455, MTCY21B4.28c, Rv1411c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mha OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mha RCSB], [http://www.ebi.ac.uk/pdbsum/3mha PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mh/3mha_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Knockout of lprG results in decreased virulence of Mycobacterium tuberculosis (MTB) in mice. MTB lipoprotein LprG has TLR2 agonist activity, which is thought to be dependent on its N-terminal triacylation. Unexpectedly, here we find that nonacylated LprG retains TLR2 activity. Moreover, we show LprG association with triacylated glycolipid TLR2 agonists lipoarabinomannan, lipomannan and phosphatidylinositol mannosides (which share core structures). Binding of triacylated species was specific to LprG (not LprA) and increased LprG TLR2 agonist activity; conversely, association of glycolipids with LprG enhanced their recognition by TLR2. The crystal structure of LprG in complex with phosphatidylinositol mannoside revealed a hydrophobic pocket that accommodates the three alkyl chains of the ligand. In conclusion, we demonstrate a glycolipid binding function of LprG that enhances recognition of triacylated MTB glycolipids by TLR2 and may affect glycolipid assembly or transport for bacterial cell wall biogenesis. | ||
| - | + | Mycobacterium tuberculosis lipoprotein LprG (Rv1411c) binds triacylated glycolipid agonists of Toll-like receptor 2.,Drage MG, Tsai HC, Pecora ND, Cheng TY, Arida AR, Shukla S, Rojas RE, Seshadri C, Moody DB, Boom WH, Sacchettini JC, Harding CV Nat Struct Mol Biol. 2010 Aug 8. PMID:20694006<ref>PMID:20694006</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
| - | [[Category: Sacchettini, J C | + | [[Category: Sacchettini, J C]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: Tsai, H C | + | [[Category: Tsai, H C]] |
[[Category: Glycolipid binding protein]] | [[Category: Glycolipid binding protein]] | ||
[[Category: Lipid binding protein]] | [[Category: Lipid binding protein]] | ||
[[Category: Lipoprotein]] | [[Category: Lipoprotein]] | ||
[[Category: Lprg]] | [[Category: Lprg]] | ||
| - | [[Category: Structural genomic]] | ||
| - | [[Category: Tb structural genomics consortium]] | ||
[[Category: Tbsgc]] | [[Category: Tbsgc]] | ||
Revision as of 16:20, 18 December 2014
Crystal structure of LprG from Mycobacterium tuberculosis bound to PIM
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