1dyq
From Proteopedia
| Line 5: | Line 5: | ||
==Overview== | ==Overview== | ||
| - | Staphylococcal enterotoxins (SEs) are superantigenic protein toxins, responsible for a number of life-threatening diseases. The X-ray structure, of a staphylococcal enterotoxin A (SEA) triple-mutant (L48R, D70R, and, Y92A) vaccine reveals a cascade of structural rearrangements located in, three loop regions essential for binding the alpha subunit of major, histocompatibility complex class II (MHC-II) molecules. A comparison of, hypothetical model complexes between SEA and the SEA triple mutant with, MHC-II HLA-DR1 clearly shows disruption of key ionic and hydrophobic, interactions necessary for forming the complex. Extensive dislocation of, the disulfide loop in particular interferes with MHC-IIalpha binding. The, triple-mutant structure provides new insights into the loss of, . | + | Staphylococcal enterotoxins (SEs) are superantigenic protein toxins, responsible for a number of life-threatening diseases. The X-ray structure, of a staphylococcal enterotoxin A (SEA) triple-mutant (L48R, D70R, and, Y92A) vaccine reveals a cascade of structural rearrangements located in, three loop regions essential for binding the alpha subunit of major, histocompatibility complex class II (MHC-II) molecules. A comparison of, hypothetical model complexes between SEA and the SEA triple mutant with, MHC-II HLA-DR1 clearly shows disruption of key ionic and hydrophobic, interactions necessary for forming the complex. Extensive dislocation of, the disulfide loop in particular interferes with MHC-IIalpha binding. The, triple-mutant structure provides new insights into the loss of, superantigenicity and toxicity of an engineered superantigen and provides, a basis for further design of enterotoxin vaccines. |
==About this Structure== | ==About this Structure== | ||
| - | 1DYQ is a | + | 1DYQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with ZN and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: ZN1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DYQ OCA]. |
==Reference== | ==Reference== | ||
| Line 23: | Line 23: | ||
[[Category: vaccine]] | [[Category: vaccine]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 12:37:02 2007'' |
Revision as of 10:31, 5 November 2007
|
STAPHYLOCOCCAL ENTEROTOXIN A MUTANT VACCINE
Overview
Staphylococcal enterotoxins (SEs) are superantigenic protein toxins, responsible for a number of life-threatening diseases. The X-ray structure, of a staphylococcal enterotoxin A (SEA) triple-mutant (L48R, D70R, and, Y92A) vaccine reveals a cascade of structural rearrangements located in, three loop regions essential for binding the alpha subunit of major, histocompatibility complex class II (MHC-II) molecules. A comparison of, hypothetical model complexes between SEA and the SEA triple mutant with, MHC-II HLA-DR1 clearly shows disruption of key ionic and hydrophobic, interactions necessary for forming the complex. Extensive dislocation of, the disulfide loop in particular interferes with MHC-IIalpha binding. The, triple-mutant structure provides new insights into the loss of, superantigenicity and toxicity of an engineered superantigen and provides, a basis for further design of enterotoxin vaccines.
About this Structure
1DYQ is a Single protein structure of sequence from Staphylococcus aureus with ZN and SO4 as ligands. Structure known Active Site: ZN1. Full crystallographic information is available from OCA.
Reference
Structural basis for abrogated binding between staphylococcal enterotoxin A superantigen vaccine and MHC-IIalpha., Krupka HI, Segelke BW, Ulrich RG, Ringhofer S, Knapp M, Rupp B, Protein Sci. 2002 Mar;11(3):642-51. PMID:11847286
Page seeded by OCA on Mon Nov 5 12:37:02 2007
