1qj8
From Proteopedia
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| - | [[Image:1qj8.jpg|left|200px]] | + | [[Image:1qj8.jpg|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX FROM ESCHERICHIA COLI''' | + | {{Structure |
| + | |PDB= 1qj8 |SIZE=350|CAPTION= <scene name='initialview01'>1qj8</scene>, resolution 1.9Å | ||
| + | |SITE= <scene name='pdbsite=PC1:Ptcl2-Binding+Site'>PC1</scene> and <scene name='pdbsite=PC2:Ptcl2-Binding+Site'>PC2</scene> | ||
| + | |LIGAND= <scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene> and <scene name='pdbligand=PCL:PLATINUM(II) DI-CHLORIDE'>PCL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= OMPX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX FROM ESCHERICHIA COLI''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1QJ8 is a [ | + | 1QJ8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QJ8 OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of the outer membrane protein OmpX from Escherichia coli reveals possible mechanisms of virulence., Vogt J, Schulz GE, Structure. 1999 Oct 15;7(10):1301-9. PMID:[http:// | + | The structure of the outer membrane protein OmpX from Escherichia coli reveals possible mechanisms of virulence., Vogt J, Schulz GE, Structure. 1999 Oct 15;7(10):1301-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10545325 10545325] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: platinum complex structure]] | [[Category: platinum complex structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:38:26 2008'' |
Revision as of 11:38, 20 March 2008
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| , resolution 1.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | and | ||||||
| Ligands: | and | ||||||
| Gene: | OMPX (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE OUTER MEMBRANE PROTEIN OMPX FROM ESCHERICHIA COLI
Overview
BACKGROUND: The integral outer membrane protein X (OmpX) from Escherichia coli belongs to a family of highly conserved bacterial proteins that promote bacterial adhesion to and entry into mammalian cells. Moreover, these proteins have a role in the resistance against attack by the human complement system. Here we present the first crystal structure of a member of this family. RESULTS: The crystal structure of OmpX from E. coli was determined at 1.9 A resolution using multiple isomorphous replacement. OmpX consists of an eight-stranded antiparallel all-next-neighbor beta barrel. The structure shows two girdles of aromatic amino acid residues and a ribbon of nonpolar residues that attach to the membrane interior. The core of the barrel consists of an extended hydrogen-bonding network of highly conserved residues. OmpX thus resembles an inverse micelle. The structure explains the dramatically improved crystal quality of OmpX containing the mutation His100-->Asn, which made the X-ray analysis possible. The coordination spheres of two bound platinum ions are described. CONCLUSIONS: The OmpX structure shows that within a family of virulence-related membrane proteins, the membrane-spanning part of the protein is much better conserved than the extracellular loops. Moreover, these loops form a protruding beta sheet, the edge of which presumably binds to external proteins. It is suggested that this type of binding promotes cell adhesion and invasion and helps defend against the complement system. Although OmpX has the same beta-sheet topology as the structurally related outer membrane protein A (OmpA), their barrels differ with respect to the shear numbers and internal hydrogen-bonding networks.
About this Structure
1QJ8 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The structure of the outer membrane protein OmpX from Escherichia coli reveals possible mechanisms of virulence., Vogt J, Schulz GE, Structure. 1999 Oct 15;7(10):1301-9. PMID:10545325
Page seeded by OCA on Thu Mar 20 13:38:26 2008
