1qk1
From Proteopedia
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| - | [[Image:1qk1.gif|left|200px]] | + | [[Image:1qk1.gif|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF HUMAN UBIQUITOUS MITOCHONDRIAL CREATINE KINASE''' | + | {{Structure |
| + | |PDB= 1qk1 |SIZE=350|CAPTION= <scene name='initialview01'>1qk1</scene>, resolution 2.70Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Creatine_kinase Creatine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.3.2 2.7.3.2] | ||
| + | |GENE= GENBANK ACCESSION J04469 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF HUMAN UBIQUITOUS MITOCHONDRIAL CREATINE KINASE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1QK1 is a [ | + | 1QK1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK1 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of human ubiquitous mitochondrial creatine kinase., Eder M, Fritz-Wolf K, Kabsch W, Wallimann T, Schlattner U, Proteins. 2000 May 15;39(3):216-25. PMID:[http:// | + | Crystal structure of human ubiquitous mitochondrial creatine kinase., Eder M, Fritz-Wolf K, Kabsch W, Wallimann T, Schlattner U, Proteins. 2000 May 15;39(3):216-25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10737943 10737943] |
[[Category: Creatine kinase]] | [[Category: Creatine kinase]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: octamer stability]] | [[Category: octamer stability]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:38:38 2008'' |
Revision as of 11:38, 20 March 2008
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| , resolution 2.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | GENBANK ACCESSION J04469 (Homo sapiens) | ||||||
| Activity: | Creatine kinase, with EC number 2.7.3.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF HUMAN UBIQUITOUS MITOCHONDRIAL CREATINE KINASE
Overview
Creatine kinase (CK), catalyzing the reversible trans-phosphorylation between ATP and creatine, plays a key role in the energy metabolism of cells with high and fluctuating energy requirements. We have solved the X-ray structure of octameric human ubiquitous mitochondrial CK (uMtCK) at 2.7 A resolution, representing the first human CK structure. The structure is very similar to the previously determined structure of sarcomeric mitochondrial CK (sMtCK). The cuboidal octamer has 422 point group symmetry with four dimers arranged along the fourfold axis and a central channel of approximately 20 A diameter, which extends through the whole octamer. Structural differences with respect to sMtCK are found in isoform-specific regions important for octamer formation and membrane binding. Octameric uMtCK is stabilized by numerous additional polar interactions between the N-termini of neighboring dimers, which extend into the central channel and form clamp-like structures, and by a pair of salt bridges in the hydrophobic interaction patch. The five C-terminal residues of uMtCK, carrying positive charges likely to be involved in phospholipid-binding, are poorly defined by electron density, indicating a more flexible region than the corresponding one in sMtCK. The structural differences between uMtCK and sMtCK are consistent with biochemical studies on octamer stability and membrane binding of the two isoforms.
About this Structure
1QK1 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of human ubiquitous mitochondrial creatine kinase., Eder M, Fritz-Wolf K, Kabsch W, Wallimann T, Schlattner U, Proteins. 2000 May 15;39(3):216-25. PMID:10737943
Page seeded by OCA on Thu Mar 20 13:38:38 2008
