1qk6
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1qk6.gif|left|200px]] | + | [[Image:1qk6.gif|left|200px]] |
| - | + | ||
| - | '''SOLUTION STRUCTURE OF HUWENTOXIN-I BY NMR''' | + | {{Structure |
| + | |PDB= 1qk6 |SIZE=350|CAPTION= <scene name='initialview01'>1qk6</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''SOLUTION STRUCTURE OF HUWENTOXIN-I BY NMR''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1QK6 is a [ | + | 1QK6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Ornithoctonus_huwena Ornithoctonus huwena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK6 OCA]. |
==Reference== | ==Reference== | ||
| - | Proton nuclear magnetic resonance studies on huwentoxin-I from the venom of the spider Selenocosmia huwena: 2. Three-dimensional structure in solution., Qu Y, Liang S, Ding J, Liu X, Zhang R, Gu X, J Protein Chem. 1997 Aug;16(6):565-74. PMID:[http:// | + | Proton nuclear magnetic resonance studies on huwentoxin-I from the venom of the spider Selenocosmia huwena: 2. Three-dimensional structure in solution., Qu Y, Liang S, Ding J, Liu X, Zhang R, Gu X, J Protein Chem. 1997 Aug;16(6):565-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9263120 9263120] |
[[Category: Ornithoctonus huwena]] | [[Category: Ornithoctonus huwena]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 24: | Line 33: | ||
[[Category: toxin]] | [[Category: toxin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:38:41 2008'' |
Revision as of 11:38, 20 March 2008
| |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF HUWENTOXIN-I BY NMR
Overview
The three-dimensional structure in aqueous solution of native huwentoxin-I, a neurotoxin from the venom of the spider Selenocosmia huwena, has been determined from two-dimensional H NMR data recorded at 500 and 600 MHz. Structural constraints consisting of interproton distances inferred from NOEs and dihedral angles from spin-spin coupling constants were used as input for distance geometry calculation with the program XPLOR 3.1. The best 10 structures have NOE violations < 0.3 A, dihedral violations < 2 degrees, and pairwise root-mean-square differences of 1.08 (+/- 0.20) A over backbone atoms (N, C alpha, C). The molecule adopts a compact structure consisting of a small triple-stranded antiparallel beta-sheet and five beta-turns. A small hydrophobic patch consisting of Phe 6, Trp 28, and Trp 31 is located on one side of the molecule. All six lysine residues are distributed on the molecular surface. The three disulfide bridges are buried within the molecule. The structure contains an "inhibitor cystine knot motif" which is adopted by several other small proteins, such as omega-conotoxin, agatoxin IVA, and gurmarin.
About this Structure
1QK6 is a Single protein structure of sequence from Ornithoctonus huwena. Full crystallographic information is available from OCA.
Reference
Proton nuclear magnetic resonance studies on huwentoxin-I from the venom of the spider Selenocosmia huwena: 2. Three-dimensional structure in solution., Qu Y, Liang S, Ding J, Liu X, Zhang R, Gu X, J Protein Chem. 1997 Aug;16(6):565-74. PMID:9263120
Page seeded by OCA on Thu Mar 20 13:38:41 2008
Categories: Ornithoctonus huwena | Single protein | Ding, J. | Gu, X. | Liang, S. | Liu, X. | Qu, Y. | Zhang, R. | Cystine knot | Huwentoxin-i | Spider | Toxin
