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1qko
From Proteopedia
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| - | [[Image:1qko.jpg|left|200px]] | + | [[Image:1qko.jpg|left|200px]] |
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| - | '''HIGH RESOLUTION X-RAY STRUCTURE OF AN EARLY INTERMEDIATE IN THE BACTERIORHODOPSIN PHOTOCYCLE''' | + | {{Structure |
| + | |PDB= 1qko |SIZE=350|CAPTION= <scene name='initialview01'>1qko</scene>, resolution 2.10Å | ||
| + | |SITE= <scene name='pdbsite=SFF:Schiff+Base'>SFF</scene> | ||
| + | |LIGAND= <scene name='pdbligand=RET:RETINAL'>RET</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''HIGH RESOLUTION X-RAY STRUCTURE OF AN EARLY INTERMEDIATE IN THE BACTERIORHODOPSIN PHOTOCYCLE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1QKO is a [ | + | 1QKO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QKO OCA]. |
==Reference== | ==Reference== | ||
| - | High-resolution X-ray structure of an early intermediate in the bacteriorhodopsin photocycle., Edman K, Nollert P, Royant A, Belrhali H, Pebay-Peyroula E, Hajdu J, Neutze R, Landau EM, Nature. 1999 Oct 21;401(6755):822-6. PMID:[http:// | + | High-resolution X-ray structure of an early intermediate in the bacteriorhodopsin photocycle., Edman K, Nollert P, Royant A, Belrhali H, Pebay-Peyroula E, Hajdu J, Neutze R, Landau EM, Nature. 1999 Oct 21;401(6755):822-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10548112 10548112] |
[[Category: Halobacterium salinarum]] | [[Category: Halobacterium salinarum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: RET]] | [[Category: RET]] | ||
[[Category: intermediate state]] | [[Category: intermediate state]] | ||
| - | [[Category: lipidic cubic | + | [[Category: lipidic cubic phase]] |
[[Category: membrane protein]] | [[Category: membrane protein]] | ||
[[Category: photocycle]] | [[Category: photocycle]] | ||
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[[Category: retinal protein]] | [[Category: retinal protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:38:55 2008'' |
Revision as of 11:38, 20 March 2008
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| , resolution 2.10Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
HIGH RESOLUTION X-RAY STRUCTURE OF AN EARLY INTERMEDIATE IN THE BACTERIORHODOPSIN PHOTOCYCLE
Overview
Bacteriorhodopsin is the simplest known photon-driven proton pump and as such provides a model for the study of a basic function in bioenergetics. Its seven transmembrane helices encompass a proton translocation pathway containing the chromophore, a retinal molecule covalently bound to lysine 216 through a protonated Schiff base, and a series of proton donors and acceptors. Photoisomerization of the all-trans retinal to the 13-cis configuration initiates the vectorial translocation of a proton from the Schiff base, the primary proton donor, to the extracellular side, followed by reprotonation of the Schiff base from the cytoplasm. Here we describe the high-resolution X-ray structure of an early intermediate in the photocycle of bacteriorhodopsin, which is formed directly after photoexcitation. A key water molecule is dislocated, allowing the primary proton acceptor, Asp 85, to move. Movement of the main-chain Lys 216 locally disrupts the hydrogen-bonding network of helix G, facilitating structural changes later in the photocycle.
About this Structure
1QKO is a Single protein structure of sequence from Halobacterium salinarum. Full crystallographic information is available from OCA.
Reference
High-resolution X-ray structure of an early intermediate in the bacteriorhodopsin photocycle., Edman K, Nollert P, Royant A, Belrhali H, Pebay-Peyroula E, Hajdu J, Neutze R, Landau EM, Nature. 1999 Oct 21;401(6755):822-6. PMID:10548112
Page seeded by OCA on Thu Mar 20 13:38:55 2008
