1ql0
From Proteopedia
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| - | [[Image:1ql0.jpg|left|200px]] | + | [[Image:1ql0.jpg|left|200px]] |
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| - | '''SM ENDONUCLEASE FROM SERATIA MARCENSCENS AT ATOMIC RESOLUTION''' | + | {{Structure |
| + | |PDB= 1ql0 |SIZE=350|CAPTION= <scene name='initialview01'>1ql0</scene>, resolution 1.1Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Serratia_marcescens_nuclease Serratia marcescens nuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.30.2 3.1.30.2] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''SM ENDONUCLEASE FROM SERATIA MARCENSCENS AT ATOMIC RESOLUTION''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1QL0 is a [ | + | 1QL0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QL0 OCA]. |
==Reference== | ==Reference== | ||
| - | Atomic structure of the Serratia marcescens endonuclease at 1.1 A resolution and the enzyme reaction mechanism., Shlyapnikov SV, Lunin VV, Perbandt M, Polyakov KM, Lunin VY, Levdikov VM, Betzel C, Mikhailov AM, Acta Crystallogr D Biol Crystallogr. 2000 May;56(Pt 5):567-72. PMID:[http:// | + | Atomic structure of the Serratia marcescens endonuclease at 1.1 A resolution and the enzyme reaction mechanism., Shlyapnikov SV, Lunin VV, Perbandt M, Polyakov KM, Lunin VY, Levdikov VM, Betzel C, Mikhailov AM, Acta Crystallogr D Biol Crystallogr. 2000 May;56(Pt 5):567-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10771425 10771425] |
[[Category: Serratia marcescens]] | [[Category: Serratia marcescens]] | ||
[[Category: Serratia marcescens nuclease]] | [[Category: Serratia marcescens nuclease]] | ||
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[[Category: signal]] | [[Category: signal]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:39:04 2008'' |
Revision as of 11:39, 20 March 2008
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| , resolution 1.1Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Serratia marcescens nuclease, with EC number 3.1.30.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SM ENDONUCLEASE FROM SERATIA MARCENSCENS AT ATOMIC RESOLUTION
Overview
The three-dimensional crystal structure of Serratia marcescens endonuclease has been refined at 1.1 A resolution to an R factor of 12.9% and an R(free) of 15.6% with the use of anisotropic temperature factors. The model contains 3694 non-H atoms, 715 water molecules, four sulfate ions and two Mg(2+)-binding sites at the active sites of the homodimeric protein. It is shown that the magnesium ion linked to the active-site Asn119 of each monomer is surrounded by five water molecules and shows an octahedral coordination geometry. The temperature factors for the bound Mg(2+) ions in the A and B subunits are 7.08 and 4.60 A(2), respectively, and the average temperature factors for the surrounding water molecules are 12.13 and 10.3 A(2), respectively. In comparison with earlier structures, alternative side-chain conformations are defined for 51 residues of the dimer, including the essential active-site residue Arg57. A plausible mechanism of enzyme function is proposed based on the high-resolution S. marcescens nuclease structure, the functional characteristics of the natural and mutational forms of the enzyme and consideration of its structural analogy with homing endo-nuclease I-PpoI.
About this Structure
1QL0 is a Single protein structure of sequence from Serratia marcescens. Full crystallographic information is available from OCA.
Reference
Atomic structure of the Serratia marcescens endonuclease at 1.1 A resolution and the enzyme reaction mechanism., Shlyapnikov SV, Lunin VV, Perbandt M, Polyakov KM, Lunin VY, Levdikov VM, Betzel C, Mikhailov AM, Acta Crystallogr D Biol Crystallogr. 2000 May;56(Pt 5):567-72. PMID:10771425
Page seeded by OCA on Thu Mar 20 13:39:04 2008
