3nku

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{{STRUCTURE_3nku| PDB=3nku | SCENE= }}
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==Crystal structure of the N-terminal domain of DrrA/SidM from Legionella pneumophila==
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===Crystal structure of the N-terminal domain of DrrA/SidM from Legionella pneumophila===
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<StructureSection load='3nku' size='340' side='right' caption='[[3nku]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20651120}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3nku]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila Legionella pneumophila subsp. pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NKU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NKU FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DrrA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=91891 Legionella pneumophila subsp. pneumophila])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nku OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nku RCSB], [http://www.ebi.ac.uk/pdbsum/3nku PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In the course of Legionnaires' disease, the bacterium Legionella pneumophila affects the intracellular vesicular trafficking of infected eukaryotic cells by recruiting the small guanosine triphosphatase (GTPase) Rab1 to the cytosolic face of the Legionella-containing vacuole. In order to accomplish this, the Legionella protein DrrA contains a specific guanine nucleotide exchange activity for Rab1 activation that exchanges guanosine triphosphate (GTP) for guanosine diphosphate on Rab1. We found that the amino-terminal domain of DrrA possesses adenosine monophosphorylation (AMPylation) activity toward the switch II region of Rab1b, leading to posttranslational covalent modification of tyrosine 77. AMPylation of switch II by DrrA restricts the access of GTPase activating proteins, thereby rendering Rab1b constitutively active.
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==Function==
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The Legionella effector protein DrrA AMPylates the membrane traffic regulator Rab1b.,Muller MP, Peters H, Blumer J, Blankenfeldt W, Goody RS, Itzen A Science. 2010 Aug 20;329(5994):946-9. Epub 2010 Jul 22. PMID:20651120<ref>PMID:20651120</ref>
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[[http://www.uniprot.org/uniprot/Q29ST3_LEGPN Q29ST3_LEGPN]] Virulence effector that plays a key role in hijacking the host vesicular trafficking by recruiting the small guanosine triphosphatase (GTPase) Rab1 to the cytosolic face of the Legionella-containing vacuole (LCVs). Acts as a GDP-GTP exchange factor (GEF) for the small GTPase Rab1 (RAB1A, RAB1B or RAB1C), thereby converting Rab1 to an active GTP-bound state, leading to the incorporation of Rab1 into LCVs. Also shows RabGDI displacement factor (GDF) activity; however, this probably represents a passive activity following the GEF activity. Also acts as an adenylyltransferase by mediating the addition of adenosine 5'-monophosphate (AMP) to 'Tyr-77' of host RAB1B, thereby rendering RAB1B constitutively active. Also has adenylyltransferase activity towards Rab6 and Rab35. Also displays guanylyltransferase activity by mediating the addition of guanosine 5'-monophosphate (GMP) to host RAB1B in vitro; however such activity remains uncertain in vivo. Specifically binds phosphatidylinositol 4-phosphate (PtdIns(4)P) lipids on the cytosolic surface of the phagosomal membrane shortly after infection.<ref>PMID:16906144</ref> <ref>PMID:17952054</ref> <ref>PMID:20651120</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3nku]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila Legionella pneumophila subsp. pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NKU OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020651120</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Legionella pneumophila subsp. pneumophila]]
[[Category: Legionella pneumophila subsp. pneumophila]]
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[[Category: Blankenfeldt, W.]]
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[[Category: Blankenfeldt, W]]
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[[Category: Goody, R S.]]
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[[Category: Goody, R S]]
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[[Category: Itzen, A.]]
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[[Category: Itzen, A]]
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[[Category: Mueller, M P.]]
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[[Category: Mueller, M P]]
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[[Category: Peters, H.]]
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[[Category: Peters, H]]
[[Category: Adenylylation]]
[[Category: Adenylylation]]
[[Category: Ampylation]]
[[Category: Ampylation]]

Revision as of 17:00, 18 December 2014

Crystal structure of the N-terminal domain of DrrA/SidM from Legionella pneumophila

3nku, resolution 2.10Å

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