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3ldy
From Proteopedia
(Difference between revisions)
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| - | + | ==An extraordinary mechanism of DNA perturbation exhibited by the rare-cutting HNH restriction endonuclease PacI== | |
| - | + | <StructureSection load='3ldy' size='340' side='right' caption='[[3ldy]], [[Resolution|resolution]] 1.97Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3ldy]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_alcaligenes Pseudomonas alcaligenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LDY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LDY FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3m7k|3m7k]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pacIR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=43263 Pseudomonas alcaligenes])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ldy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ldy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ldy RCSB], [http://www.ebi.ac.uk/pdbsum/3ldy PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The crystal structure of the rare-cutting HNH restriction endonuclease PacI in complex with its eight-base-pair target recognition sequence 5'-TTAATTAA-3' has been determined to 1.9 A resolution. The enzyme forms an extended homodimer, with each subunit containing two zinc-bound motifs surrounding a betabetaalpha-metal catalytic site. The latter is unusual in that a tyrosine residue likely initiates strand cleavage. PacI dramatically distorts its target sequence from Watson-Crick duplex DNA base pairing, with every base separated from its original partner. Two bases on each strand are unpaired, four are engaged in noncanonical A:A and T:T base pairs, and the remaining two bases are matched with new Watson-Crick partners. This represents a highly unusual DNA binding mechanism for a restriction endonuclease, and implies that initial recognition of the target site might involve significantly different contacts from those visualized in the DNA-bound cocrystal structures. | ||
| - | + | Unusual target site disruption by the rare-cutting HNH restriction endonuclease PacI.,Shen BW, Heiter DF, Chan SH, Wang H, Xu SY, Morgan RD, Wilson GG, Stoddard BL Structure. 2010 Jun 9;18(6):734-43. PMID:20541511<ref>PMID:20541511</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Endonuclease|Endonuclease]] | *[[Endonuclease|Endonuclease]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Pseudomonas alcaligenes]] | [[Category: Pseudomonas alcaligenes]] | ||
| - | [[Category: Chan, S H | + | [[Category: Chan, S H]] |
| - | [[Category: Heiter, D | + | [[Category: Heiter, D]] |
| - | [[Category: Shen, B W | + | [[Category: Shen, B W]] |
| - | [[Category: Stoddard, B L | + | [[Category: Stoddard, B L]] |
| - | [[Category: Wilson, G | + | [[Category: Wilson, G]] |
| - | [[Category: Xu, S Y | + | [[Category: Xu, S Y]] |
[[Category: A-a]] | [[Category: A-a]] | ||
[[Category: And t-t base-pair]] | [[Category: And t-t base-pair]] | ||
Revision as of 17:09, 18 December 2014
An extraordinary mechanism of DNA perturbation exhibited by the rare-cutting HNH restriction endonuclease PacI
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