3l3f
From Proteopedia
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| - | + | ==Crystal structure of a PFU-PUL domain pair of Saccharomyces cerevisiae Doa1/Ufd3== | |
| - | + | <StructureSection load='3l3f' size='340' side='right' caption='[[3l3f]], [[Resolution|resolution]] 1.90Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3l3f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L3F FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3l3f RCSB], [http://www.ebi.ac.uk/pdbsum/3l3f PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Doa1/Ufd3 is involved in ubiquitin (Ub)-dependent cellular processes in Saccharomyces cerevisiae, and consists of WD40, PFU, and PUL domains. Previous studies showed that the PFU and PUL domains interact with Ub and Hse1, and Cdc48, respectively. However, their detailed functional interactions with Doa1 remained elusive. We report the crystal structure of the PFU-PUL domain pair of yeast Doa1 at 1.9 A resolution. The conserved surface of the PFU domain may be involved in binding to Ub and Hse1. Unexpectedly, the PUL domain consists of an Armadillo (ARM)-like repeat structure. The positively charged concave surface of the PUL domain may bind to the negatively charged C-terminal region of Cdc48. A structural comparison of Doa1 with Ufd2 revealed that they share a similar ARM-like repeat, supporting a model in which Doa1 and Ufd2 compete for Cdc48 binding and may dictate the fate of ubiquitinated proteins in the proteasome pathway. | ||
| - | + | Crystal Structure of a PFU-PUL Domain Pair of Saccharomyces Cerevisiae Doa1/Ufd3.,Nishimasu R, Komori H, Higuchi Y, Nishimasu H, Hiroaki H Kobe J Med Sci. 2010 Oct 21;56(3):E125-39. PMID:21063153<ref>PMID:21063153</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Higuchi, Y | + | [[Category: Higuchi, Y]] |
| - | [[Category: Komori, H | + | [[Category: Komori, H]] |
| - | [[Category: Kuno, T | + | [[Category: Kuno, T]] |
| - | [[Category: Nishimasu, R | + | [[Category: Nishimasu, R]] |
[[Category: Armadillo-like repeat structure]] | [[Category: Armadillo-like repeat structure]] | ||
[[Category: Nucleus]] | [[Category: Nucleus]] | ||
[[Category: Protein binding]] | [[Category: Protein binding]] | ||
[[Category: Ubl conjugation pathway]] | [[Category: Ubl conjugation pathway]] | ||
Revision as of 17:14, 18 December 2014
Crystal structure of a PFU-PUL domain pair of Saccharomyces cerevisiae Doa1/Ufd3
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