3lcu

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{{STRUCTURE_3lcu| PDB=3lcu | SCENE= }}
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==Crystal Structure of Antibiotic related Methyltransferase==
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===Crystal Structure of Antibiotic related Methyltransferase===
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<StructureSection load='3lcu' size='340' side='right' caption='[[3lcu]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20194115}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3lcu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Micromonospora_zionensis Micromonospora zionensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LCU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LCU FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3lcv|3lcv]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lcu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lcu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3lcu RCSB], [http://www.ebi.ac.uk/pdbsum/3lcu PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lc/3lcu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Sgm (Sisomicin-gentamicin methyltransferase) from antibiotic-producing bacterium Micromonospora zionensis is an enzyme that confers resistance to aminoglycosides like gentamicin and sisomicin by specifically methylating G1405 in bacterial 16S rRNA. Sgm belongs to the aminoglycoside resistance methyltransferase (Arm) family of enzymes that have been recently found to spread by horizontal gene transfer among disease-causing bacteria. Structural characterization of Arm enzymes is the key to understand their mechanism of action and to develop inhibitors that would block their activity. Here we report the structure of Sgm in complex with cofactors S-adenosylmethionine (AdoMet) and S-adenosylhomocysteine (AdoHcy) at 2.0 and 2.1 A resolution, respectively, and results of mutagenesis and rRNA footprinting, and protein-substrate docking. We propose the mechanism of methylation of G1405 by Sgm and compare it with other m(7)G methyltransferases, revealing a surprising diversity of active sites and binding modes for the same basic reaction of RNA modification. This analysis can serve as a stepping stone towards developing drugs that would specifically block the activity of Arm methyltransferases and thereby re-sensitize pathogenic bacteria to aminoglycoside antibiotics.
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==Function==
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Structural basis for the methylation of G1405 in 16S rRNA by aminoglycoside resistance methyltransferase Sgm from an antibiotic producer: a diversity of active sites in m7G methyltransferases.,Husain N, Tkaczuk KL, Tulsidas SR, Kaminska KH, Cubrilo S, Maravic-Vlahovicek G, Bujnicki JM, Sivaraman J Nucleic Acids Res. 2010 Jul 1;38(12):4120-32. Epub 2010 Mar 1. PMID:20194115<ref>PMID:20194115</ref>
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[[http://www.uniprot.org/uniprot/Q7M0R2_9ACTO Q7M0R2_9ACTO]] Specifically methylates the N(7) position of guanine 1405 in 16S rRNA. Confers resistance to various aminoglycosides, including gentamicin, kanamycin and sisomicin.<ref>PMID:1447159</ref> <ref>PMID:19589804</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3lcu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Micromonospora_zionensis Micromonospora zionensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LCU OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020194115</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Micromonospora zionensis]]
[[Category: Micromonospora zionensis]]
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[[Category: Husain, N.]]
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[[Category: Husain, N]]
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[[Category: Sivaraman, J.]]
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[[Category: Sivaraman, J]]
[[Category: Antibiotic resistance]]
[[Category: Antibiotic resistance]]
[[Category: Methyltransferase]]
[[Category: Methyltransferase]]
[[Category: Transferase]]
[[Category: Transferase]]

Revision as of 17:16, 18 December 2014

Crystal Structure of Antibiotic related Methyltransferase

3lcu, resolution 2.10Å

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