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3lzw

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{{STRUCTURE_3lzw| PDB=3lzw | SCENE= }}
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==Crystal structure of ferredoxin-NADP+ oxidoreductase from bacillus subtilis (form I)==
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===Crystal structure of ferredoxin-NADP+ oxidoreductase from bacillus subtilis (form I)===
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<StructureSection load='3lzw' size='340' side='right' caption='[[3lzw]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20878669}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3lzw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LZW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LZW FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3lzx|3lzx]]</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lzw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3lzw RCSB], [http://www.ebi.ac.uk/pdbsum/3lzw PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacillus subtilis yumC encodes a novel type of ferredoxin-NADP(+) oxidoreductase (FNR) with a primary sequence and oligomeric conformation distinct from those of previously known FNRs. In this study, the crystal structure of B. subtilis FNR (BsFNR) complexed with NADP(+) has been determined. BsFNR features two distinct binding domains for FAD and NADPH in accordance with its structural similarity to Escherichia coli NADPH-thioredoxin reductase (TdR) and TdR-like protein from Thermus thermophilus HB8 (PDB code: 2ZBW). The deduced mode of NADP(+) binding to the BsFNR molecule is nonproductive in that the nicotinamide and isoalloxazine rings are over 15 A apart. A unique C-terminal extension, not found in E. coli TdR but in TdR-like protein from T. thermophilus HB8, covers the re-face of the isoalloxazine moiety of FAD. In particular, Tyr50 in the FAD-binding region and His324 in the C-terminal extension stack on the si- and re-faces of the isoalloxazine ring of FAD, respectively. Aromatic residues corresponding to Tyr50 and His324 are also found in the plastid-type FNR superfamily of enzymes, and the residue corresponding to His324 has been reported to be responsible for nucleotide specificity. In contrast to the plastid-type FNRs, replacement of His324 with Phe or Ser had little effect on the specificity or reactivity of BsFNR with NAD(P)H, whereas replacement of Arg190, which interacts with the 2'-phosphate of NADP(+) , drastically decreased its affinity toward NADPH. This implies that BsFNR adopts the same nucleotide binding mode as the TdR enzyme family and that aromatic residue on the re-face of FAD is hardly relevant to the nucleotide selectivity.
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==About this Structure==
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Crystal structure analysis of Bacillus subtilis ferredoxin-NADP(+) oxidoreductase and the structural basis for its substrate selectivity.,Komori H, Seo D, Sakurai T, Higuchi Y Protein Sci. 2010 Dec;19(12):2279-90. doi: 10.1002/pro.508. Epub 2010 Nov, 3. PMID:20878669<ref>PMID:20878669</ref>
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[[3lzw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LZW OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:020878669</ref><references group="xtra"/><references/>
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</div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Higuchi, Y.]]
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[[Category: Higuchi, Y]]
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[[Category: Komori, H.]]
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[[Category: Komori, H]]
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[[Category: Sakurai, T.]]
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[[Category: Sakurai, T]]
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[[Category: Seo, D.]]
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[[Category: Seo, D]]
[[Category: Fad]]
[[Category: Fad]]
[[Category: Ferredoxin reductase]]
[[Category: Ferredoxin reductase]]

Revision as of 17:18, 18 December 2014

Crystal structure of ferredoxin-NADP+ oxidoreductase from bacillus subtilis (form I)

3lzw, resolution 1.80Å

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