3kph
From Proteopedia
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| - | + | ==Crystal structure of Mycoplasma arthritidis-derived mitogen== | |
| - | === | + | <StructureSection load='3kph' size='340' side='right' caption='[[3kph]], [[Resolution|resolution]] 2.80Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3kph]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycoplasma_arthritidis Mycoplasma arthritidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KPH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KPH FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1r5i|1r5i]], [[2icw|2icw]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kph OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kph RCSB], [http://www.ebi.ac.uk/pdbsum/3kph PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Mycoplasma arthritidis-derived mitogen (MAM) is a superantigen that can activate large fractions of T cells bearing particular Vbeta elements of T cell receptor. Here, we report the crystal structure of a MAM mutant K201A in apo form (unliganded) at 2.8-A resolutions. We also partially refined the crystal structures of the MAM wild type and another MAM mutant L50A in apo forms at low resolutions. Unexpectedly, the structures of these apo MAM molecules display a three-dimensional domain-swapped dimer. The entire C-terminal domains of these MAM molecules are involved in the domain swapping. Functional analyses demonstrated that the K201A and L50A mutants do not show altered ability to bind to their host receptors and that they stimulate the activation of T cells as efficiently as does the wild type. Structural comparisons indicated that the "reconstituted" MAM monomer from the domain-swapped dimer displays large differences at the hinge regions from the MAM(wt) molecule in the receptor-bound form. Further comparison indicated that MAM has a flexible N-terminal loop, implying that conformational changes could occur upon receptor binding. | ||
| - | + | Crystal structure of the Mycoplasma arthritidis-derived mitogen in apo form reveals a 3D domain-swapped dimer.,Liu L, Li Z, Guo Y, VanVranken SJ, Mourad W, Li H J Mol Biol. 2010 Jun 11;399(3):367-76. Epub 2010 Apr 22. PMID:20417218<ref>PMID:20417218</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Mycoplasma arthritidis]] | [[Category: Mycoplasma arthritidis]] | ||
| - | [[Category: Li, H M | + | [[Category: Li, H M]] |
| - | [[Category: Liu, L H | + | [[Category: Liu, L H]] |
[[Category: 3d-domain swap]] | [[Category: 3d-domain swap]] | ||
[[Category: Immune system]] | [[Category: Immune system]] | ||
[[Category: Mam]] | [[Category: Mam]] | ||
[[Category: Superantigen]] | [[Category: Superantigen]] | ||
Revision as of 17:21, 18 December 2014
Crystal structure of Mycoplasma arthritidis-derived mitogen
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