3ljq

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "3ljq" [edit=sysop:move=sysop])
Line 1: Line 1:
-
{{STRUCTURE_3ljq| PDB=3ljq | SCENE= }}
+
==Crystal Structure of the Glycosylasparaginase T152C apo-precursor==
-
===Crystal Structure of the Glycosylasparaginase T152C apo-precursor===
+
<StructureSection load='3ljq' size='340' side='right' caption='[[3ljq]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-
{{ABSTRACT_PUBMED_20800597}}
+
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3ljq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LJQ FirstGlance]. <br>
 +
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
 +
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[9gaa|9gaa]], [[9gac|9gac]], [[9gaf|9gaf]], [[1p4v|1p4v]], [[1p4k|1p4k]]</td></tr>
 +
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GA(1-295) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=238 Elizabethkingia meningoseptica])</td></tr>
 +
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.26 3.5.1.26] </span></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ljq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ljq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ljq RCSB], [http://www.ebi.ac.uk/pdbsum/3ljq PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Glycosylasparaginase belongs to a family of N-terminal nucleophile hydrolases that autoproteolytically generate their mature enzymes from single-chain protein precursors. Previously, based on a precursor structure paused at pre-autoproteolysis stage by a reversible inhibitor (glycine), we proposed a mechanism of intramolecular autoproteolysis. A key structural feature, a highly strained conformation at the scissile peptide bond, had been identified and was hypothesized to be critical for driving autoproteolysis through an N-O acyl shift. To examine this "twist-and-break" hypothesis, we report here a 1. 9-A-resolution structure of an autoproteolysis-active precursor (a T152C mutant) that is free of inhibitor or ligand and is poised to undergo autoproteolysis. The current crystallographic study has provided direct evidence for the natural conformation of the glycosylasparaginase autocatalytic site without influence from any inhibitor or ligand. This finding has confirmed our previous proposal that conformational strain is an intrinsic feature of an active precursor.
-
==Function==
+
Crystallographic snapshot of glycosylasparaginase precursor poised for autoprocessing.,Wang Y, Guo HC J Mol Biol. 2010 Oct 15;403(1):120-30. Epub 2010 Aug 26. PMID:20800597<ref>PMID:20800597</ref>
-
[[http://www.uniprot.org/uniprot/ASPG_ELIMR ASPG_ELIMR]] Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins. Requires that the glycosylated asparagine moiety is not substituted on its N-(R1) and C- (R2) terminus.
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[3ljq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LJQ OCA].
+
</div>
-
==Reference==
+
==See Also==
-
<ref group="xtra">PMID:020800597</ref><references group="xtra"/><references/>
+
*[[Glycosylasparaginase|Glycosylasparaginase]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Elizabethkingia meningoseptica]]
[[Category: Elizabethkingia meningoseptica]]
-
[[Category: Guo, H C.]]
+
[[Category: Guo, H C]]
-
[[Category: Wang, Y.]]
+
[[Category: Wang, Y]]
[[Category: Active precursor]]
[[Category: Active precursor]]
[[Category: Aspartylglucosylaminase]]
[[Category: Aspartylglucosylaminase]]

Revision as of 17:24, 18 December 2014

Crystal Structure of the Glycosylasparaginase T152C apo-precursor

3ljq, resolution 1.90Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ljq"
Personal tools