3jzt

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{{STRUCTURE_3jzt| PDB=3jzt | SCENE= }}
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==Structure of a cubic crystal form of X (ADRP) domain from FCoV with ADP-ribose==
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===Structure of a cubic crystal form of X (ADRP) domain from FCoV with ADP-ribose===
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<StructureSection load='3jzt' size='340' side='right' caption='[[3jzt]], [[Resolution|resolution]] 3.91&Aring;' scene=''>
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{{ABSTRACT_PUBMED_19966415}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3jzt]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Feline_coronavirus Feline coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JZT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JZT FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ew5|3ew5]], [[3eti|3eti]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rep, 1a-1b ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12663 Feline coronavirus])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jzt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3jzt RCSB], [http://www.ebi.ac.uk/pdbsum/3jzt PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jz/3jzt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the X (or ADRP) domain of a pathogenic variant of feline coronavirus (FCoV) has been determined in tetragonal and cubic crystal forms to 3.1 and 2.2 A resolution, respectively. In the tetragonal crystal form, glycerol-3-phosphate was observed in the ADP-ribose-binding site. Both crystal forms contained large solvent channels and had a solvent content of higher than 70%. Only very weak binding of this domain to ADP-ribose was detected in vitro. However, the structure with ADP-ribose bound was determined in the cubic crystal form at 3.9 A resolution. The structure of the FCoV X domain had the expected macro-domain fold and is the first structure of this domain from a coronavirus belonging to subgroup 1a.
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==Function==
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Structure of the X (ADRP) domain of nsp3 from feline coronavirus.,Wojdyla JA, Manolaridis I, Snijder EJ, Gorbalenya AE, Coutard B, Piotrowski Y, Hilgenfeld R, Tucker PA Acta Crystallogr D Biol Crystallogr. 2009 Dec;65(Pt 12):1292-300. Epub, 2009 Nov 17. PMID:19966415<ref>PMID:19966415</ref>
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[[http://www.uniprot.org/uniprot/R1AB_FIPV R1AB_FIPV]] The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products. The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3 (By similarity). The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity). The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) (By similarity). The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction (By similarity). Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity). NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond (By similarity).
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3jzt]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Feline_coronavirus Feline coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JZT OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019966415</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Feline coronavirus]]
[[Category: Feline coronavirus]]
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[[Category: Manolaridis, I.]]
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[[Category: Manolaridis, I]]
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[[Category: Tucker, P A.]]
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[[Category: Tucker, P A]]
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[[Category: Wojdyla, J A.]]
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[[Category: Wojdyla, J A]]
[[Category: Adp-ribose]]
[[Category: Adp-ribose]]
[[Category: Adrp domain]]
[[Category: Adrp domain]]

Revision as of 17:31, 18 December 2014

Structure of a cubic crystal form of X (ADRP) domain from FCoV with ADP-ribose

3jzt, resolution 3.91Å

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