1qox
From Proteopedia
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| - | [[Image:1qox.gif|left|200px]] | + | [[Image:1qox.gif|left|200px]] |
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| - | '''BETA-GLUCOSIDASE FROM BACILLUS CIRCULANS SP. ALKALOPHILUS''' | + | {{Structure |
| + | |PDB= 1qox |SIZE=350|CAPTION= <scene name='initialview01'>1qox</scene>, resolution 2.7Å | ||
| + | |SITE= <scene name='pdbsite=CAA:Active+Site+(Chain+A)'>CAA</scene>, <scene name='pdbsite=CAB:Active+Site+(Chain+B)'>CAB</scene>, <scene name='pdbsite=CAC:Active+Site+(Chain+C)'>CAC</scene>, <scene name='pdbsite=CAD:Active+Site+(Chain+D)'>CAD</scene>, <scene name='pdbsite=CAE:Active+Site+(Chain+E)'>CAE</scene>, <scene name='pdbsite=CAF:Active+Site+(Chain+F)'>CAF</scene>, <scene name='pdbsite=CAG:Active+Site+(Chain+G)'>CAG</scene>, <scene name='pdbsite=CAH:Active+Site+(Chain+H)'>CAH</scene>, <scene name='pdbsite=CAI:Active+Site+(Chain+I)'>CAI</scene>, <scene name='pdbsite=CAJ:Active+Site+(Chain+J)'>CAJ</scene>, <scene name='pdbsite=CAK:Active+Site+(Chain+K)'>CAK</scene>, <scene name='pdbsite=CAL:Active+Site+(Chain+L)'>CAL</scene>, <scene name='pdbsite=CAM:Active+Site+(Chain+M)'>CAM</scene>, <scene name='pdbsite=CAN:Active+Site+(Chain+N)'>CAN</scene>, <scene name='pdbsite=CAO:Active+Site+(Chain+O)'>CAO</scene> and <scene name='pdbsite=CAP:Active+Site+(Chain+P)'>CAP</scene> | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''BETA-GLUCOSIDASE FROM BACILLUS CIRCULANS SP. ALKALOPHILUS''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1QOX is a [ | + | 1QOX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QOX OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of beta-glucosidase from Bacillus circulans sp. alkalophilus: ability to form long polymeric assemblies., Hakulinen N, Paavilainen S, Korpela T, Rouvinen J, J Struct Biol. 2000 Feb;129(1):69-79. PMID:[http:// | + | The crystal structure of beta-glucosidase from Bacillus circulans sp. alkalophilus: ability to form long polymeric assemblies., Hakulinen N, Paavilainen S, Korpela T, Rouvinen J, J Struct Biol. 2000 Feb;129(1):69-79. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10675298 10675298] |
[[Category: Bacillus circulans]] | [[Category: Bacillus circulans]] | ||
[[Category: Beta-glucosidase]] | [[Category: Beta-glucosidase]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:40:39 2008'' |
Revision as of 11:40, 20 March 2008
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| , resolution 2.7Å | |||||||
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| Sites: | , , , , , , , , , , , , , , and | ||||||
| Activity: | Beta-glucosidase, with EC number 3.2.1.21 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
BETA-GLUCOSIDASE FROM BACILLUS CIRCULANS SP. ALKALOPHILUS
Overview
Family 1 of glycosyl hydrolases is a large and biologically important group of enzymes. A new three-dimensional structure of this family, beta-glucosidase from Bacillus circulans sp. alkalophilus is reported here. This is the first structure of beta-glucosidase from an alkaliphilic organism. The model was determined by the molecular replacement method and refined to a resolution of 2.7 A. The quaternary structure of B. circulans sp. alkalophilus beta-glucosidase is an octamer and subunits of the octamer show a similar (beta/alpha)(8) barrel fold to that previously reported for other family 1 enzymes. The crystal structure suggested that Cys169 in the active site is substituted. The Cys169 is located near the putative acid/base catalyst Glu166 and it may contribute to the high pH optimum of the enzyme. The crystal structure also revealed that the asymmetric unit contains two octamers which have a clear binding interaction with each other. The ability of the octamers to link with each other suggested that beta-glucosidase from Bacillus circulans sp. alkalophilus is able to form long polymeric assemblies, at least in the crystalline state.
About this Structure
1QOX is a Single protein structure of sequence from Bacillus circulans. Full crystallographic information is available from OCA.
Reference
The crystal structure of beta-glucosidase from Bacillus circulans sp. alkalophilus: ability to form long polymeric assemblies., Hakulinen N, Paavilainen S, Korpela T, Rouvinen J, J Struct Biol. 2000 Feb;129(1):69-79. PMID:10675298
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