3k4q

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{{STRUCTURE_3k4q| PDB=3k4q | SCENE= }}
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==Aspergillus niger Phytase in complex with myo-inositol hexakis sulfate==
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===Aspergillus niger Phytase in complex with myo-inositol hexakis sulfate===
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<StructureSection load='3k4q' size='340' side='right' caption='[[3k4q]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20541524}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3k4q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K4Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K4Q FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IHS:D-MYO-INOSITOL-HEXASULPHATE'>IHS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ihp|1ihp]], [[2gfi|2gfi]], [[1dkl|1dkl]], [[1dkq|1dkq]], [[1qfx|1qfx]], [[3k4p|3k4p]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PhyA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5061 Aspergillus niger])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phytase 3-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.8 3.1.3.8] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k4q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k4q RCSB], [http://www.ebi.ac.uk/pdbsum/3k4q PDBsum]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k4/3k4q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phytases hydrolyse the phosphomonoesters of phytate (myo-inositol-1,2,3,4,5,6-hexakis phosphate) and thus find uses in plant and animal production through the mobilisation of phosphorus from this source. The structure of partially deglycosylated Aspergillus niger PhyA is presented in apo form and in complex with the potent inhibitor myo-inositol-1,2,3,4,5,6-hexakis sulfate, which by analogy with phytate provides a snapshot of the Michaelis complex. The structure explains the enzyme's preference for the 3'-phosphate of phytate. The apo-and inhibitor-bound forms are similar and no induced-fit mechanism operates. Furthermore the enzyme structure is apparently unaffected by the presence of glycosides on the surface. The new structures of A. niger PhyA are discussed in the context of protein engineering studies aimed at modulating pH preference and stability.
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==Function==
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The structure of Aspergillus niger phytase PhyA in complex with a phytate mimetic.,Oakley AJ Biochem Biophys Res Commun. 2010 Jul 9;397(4):745-9. Epub 2010 Jun 10. PMID:20541524<ref>PMID:20541524</ref>
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[[http://www.uniprot.org/uniprot/PHYA_ASPNG PHYA_ASPNG]] Catalyzes the hydrolysis of inorganic orthophosphate from phytate.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3k4q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K4Q OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020541524</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: 3-phytase]]
[[Category: 3-phytase]]
[[Category: Aspergillus niger]]
[[Category: Aspergillus niger]]
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[[Category: Oakley, A J.]]
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[[Category: Oakley, A J]]
[[Category: 3-phosphotase]]
[[Category: 3-phosphotase]]
[[Category: 37288-11-2]]
[[Category: 37288-11-2]]

Revision as of 17:45, 18 December 2014

Aspergillus niger Phytase in complex with myo-inositol hexakis sulfate

3k4q, resolution 2.20Å

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