3ngl

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{{STRUCTURE_3ngl| PDB=3ngl | SCENE= }}
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==Crystal structure of bifunctional 5,10-methylenetetrahydrofolate dehydrogenase / cyclohydrolase from Thermoplasma acidophilum==
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===Crystal structure of bifunctional 5,10-methylenetetrahydrofolate dehydrogenase / cyclohydrolase from Thermoplasma acidophilum===
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<StructureSection load='3ngl' size='340' side='right' caption='[[3ngl]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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{{ABSTRACT_PUBMED_21333632}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ngl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NGL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NGL FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ngx|3ngx]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ngl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ngl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ngl RCSB], [http://www.ebi.ac.uk/pdbsum/3ngl PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Folate co-enzymes play a pivotal role in one-carbon transfer cellular processes. Many eukaryotes encode the tri-functional tetrahydrofolate dehydrogenase/cyclohydrolase/synthetase (deh/cyc/syn) enzyme, which consists of a N-terminal bifunctional domain (deh/cyc) and a C-terminal monofunctional domain (syn). Here, we report the first analogous archeal enzyme structures, for the bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase from Thermoplasma acidophilum (TaMTHFDC) as the native protein and also as its NADP complex. The TaMTHFDC structure is a dimer with a polar interface, as well as a NADP binding site that shows minor conformational change. The orientations of the residues in the NADP binding site do not change on ligand binding, incorporating three water molecules which are hydrogen bonded with phosphate groups of NADP in the structure of the complex. Our structural information will contribute to an improved understanding of the basis of THF and one-carbon metabolism.
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==Function==
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Crystal structure of bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase from Thermoplasma acidophilum.,Lee WH, Sung MW, Kim JH, Kim YK, Han A, Hwang KY Biochem Biophys Res Commun. 2011 Mar 18;406(3):459-63. Epub 2011 Feb 17. PMID:21333632<ref>PMID:21333632</ref>
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[[http://www.uniprot.org/uniprot/FOLD_THEAC FOLD_THEAC]] Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate (By similarity).
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3ngl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NGL OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:021333632</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Thermoplasma acidophilum]]
[[Category: Thermoplasma acidophilum]]
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[[Category: Hwang, K Y.]]
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[[Category: Hwang, K Y]]
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[[Category: Lee, W H.]]
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[[Category: Lee, W H]]
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[[Category: Sung, M W.]]
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[[Category: Sung, M W]]
[[Category: 10-methenyltetrahydrofolate]]
[[Category: 10-methenyltetrahydrofolate]]
[[Category: 10-methenyltetrahydrofolate to 10-formyltetrahydrofolate]]
[[Category: 10-methenyltetrahydrofolate to 10-formyltetrahydrofolate]]

Revision as of 17:53, 18 December 2014

Crystal structure of bifunctional 5,10-methylenetetrahydrofolate dehydrogenase / cyclohydrolase from Thermoplasma acidophilum

3ngl, resolution 2.40Å

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