3o18
From Proteopedia
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| - | + | ==Crystal structure of c-phycocyanin from Themosynechococcus vulcanus at 1.35 angstroms resolution== | |
| - | + | <StructureSection load='3o18' size='340' side='right' caption='[[3o18]], [[Resolution|resolution]] 1.35Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3o18]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_vulcanus Thermosynechococcus vulcanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O18 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O18 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MEN:N-METHYL+ASPARAGINE'>MEN</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ktp|1ktp]], [[1on7|1on7]], [[1i7y|1i7y]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o18 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o18 RCSB], [http://www.ebi.ac.uk/pdbsum/3o18 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The phycobilisome light-harvesting antenna in cyanobacteria and red algae is assembled from two substructures: a central core composed of allophycocyanin surrounded by rods that always contain phycocyanin (PC). Unpigmented proteins called linkers are also found within the rods and core. We present here two new structures of PC from the thermophilic cyanobacterium Thermosynechococcus vulcanus. We have determined the structure of trimeric PC to 1.35 A, the highest resolution reported to date for this protein. We also present a structure of PC isolated in its intact and functional rod form at 1.5 A. Analysis of rod crystals showed that in addition to the alpha and beta PC subunit, there were three linker proteins: the capping rod linker (L(R)(8.7)), the rod linker (L(R)), and only one of three rod-core linkers (L(RC), CpcG4) with a stoichiometry of 12:12:1:1:1. This ratio indicates that the crystals contained rods composed of two hexamers. The crystallographic parameters of the rod crystals are nearly identical with that of the trimeric form, indicating that the linkers do not affect crystal packing and are completely embedded within the rod cavities. Absorption and fluorescence emission spectra were red-shifted, as expected for assembled rods, and this could be shown for the rod in solution as well as in crystal using confocal fluorescence microscopy. The crystal packing imparts superimposition of the three rod linkers, canceling out their electron density. However, analysis of B-factors and the conformations of residues facing the rod channel indicate the presence of linkers. Based on the experimental evidence presented here and a homology-based model of the L(R) protein, we suggest that the linkers do not in fact link between rod hexamers but stabilize the hexameric assembly and modify rod energy absorption and transfer capabilities. | ||
| - | + | High-resolution crystal structures of trimeric and rod phycocyanin.,David L, Marx A, Adir N J Mol Biol. 2011 Jan 7;405(1):201-13. Epub 2010 Oct 28. PMID:21035460<ref>PMID:21035460</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Thermosynechococcus vulcanus]] | [[Category: Thermosynechococcus vulcanus]] | ||
| - | [[Category: Adir, N | + | [[Category: Adir, N]] |
| - | [[Category: David, L | + | [[Category: David, L]] |
| - | [[Category: Marx, A | + | [[Category: Marx, A]] |
[[Category: Cyanobacteria]] | [[Category: Cyanobacteria]] | ||
[[Category: Light harvesting]] | [[Category: Light harvesting]] | ||
[[Category: Photosynthesis]] | [[Category: Photosynthesis]] | ||
[[Category: Phycobilisome]] | [[Category: Phycobilisome]] | ||
Revision as of 06:42, 19 December 2014
Crystal structure of c-phycocyanin from Themosynechococcus vulcanus at 1.35 angstroms resolution
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