3npe

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "3npe" [edit=sysop:move=sysop])
Line 1: Line 1:
-
{{STRUCTURE_3npe| PDB=3npe | SCENE= }}
+
==Structure of VP14 in complex with oxygen==
-
===Structure of VP14 in complex with oxygen===
+
<StructureSection load='3npe' size='340' side='right' caption='[[3npe]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
-
{{ABSTRACT_PUBMED_20884803}}
+
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3npe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NPE FirstGlance]. <br>
 +
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
 +
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VP14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 Zea mays])</td></tr>
 +
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/9-cis-epoxycarotenoid_dioxygenase 9-cis-epoxycarotenoid dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.51 1.13.11.51] </span></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3npe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3npe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3npe RCSB], [http://www.ebi.ac.uk/pdbsum/3npe PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The key regulatory step in the biosynthesis of abscisic acid (ABA), a hormone central to the regulation of several important processes in plants, is the oxidative cleavage of the 11,12 double bond of a 9-cis-epoxycarotenoid. The enzyme viviparous14 (VP14) performs this cleavage in maize (Zea mays), making it a target for the rational design of novel chemical agents and genetic modifications that improve plant behavior through the modulation of ABA levels. The structure of VP14, determined to 3.2-A resolution, provides both insight into the determinants of regio- and stereospecificity of this enzyme and suggests a possible mechanism for oxidative cleavage. Furthermore, mutagenesis of the distantly related CCD1 of maize shows how the VP14 structure represents a template for all plant carotenoid cleavage dioxygenases (CCDs). In addition, the structure suggests how VP14 associates with the membrane as a way of gaining access to its membrane soluble substrate.
-
==Function==
+
Structural insights into maize viviparous14, a key enzyme in the biosynthesis of the phytohormone abscisic acid.,Messing SA, Gabelli SB, Echeverria I, Vogel JT, Guan JC, Tan BC, Klee HJ, McCarty DR, Amzel LM Plant Cell. 2010 Sep;22(9):2970-80. Epub 2010 Sep 30. PMID:20884803<ref>PMID:20884803</ref>
-
[[http://www.uniprot.org/uniprot/NCED1_MAIZE NCED1_MAIZE]] Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids. Not active on the all-trans isomers of violaxanthin and neoxanthin. Contributes probably to abscisic acid synthesis for the induction of seed dormancy.<ref>PMID:9342392</ref> <ref>PMID:9188535</ref> <ref>PMID:20884803</ref>
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[3npe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NPE OCA].
+
</div>
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:020884803</ref><references group="xtra"/><references/>
+
__TOC__
 +
</StructureSection>
[[Category: 9-cis-epoxycarotenoid dioxygenase]]
[[Category: 9-cis-epoxycarotenoid dioxygenase]]
[[Category: Zea mays]]
[[Category: Zea mays]]
-
[[Category: Amzel, L M.]]
+
[[Category: Amzel, L M]]
-
[[Category: Gabelli, S B.]]
+
[[Category: Gabelli, S B]]
-
[[Category: Messing, S A.]]
+
[[Category: Messing, S A]]
[[Category: Abscisic acid]]
[[Category: Abscisic acid]]
[[Category: Dioxygenase]]
[[Category: Dioxygenase]]

Revision as of 06:54, 19 December 2014

Structure of VP14 in complex with oxygen

3npe, resolution 3.20Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3npe"
Personal tools