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3of1

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{{STRUCTURE_3of1| PDB=3of1 | SCENE= }}
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==Crystal Structure of Bcy1, the Yeast Regulatory Subunit of PKA==
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===Crystal Structure of Bcy1, the Yeast Regulatory Subunit of PKA===
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<StructureSection load='3of1' size='340' side='right' caption='[[3of1]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
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{{ABSTRACT_PUBMED_21070946}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3of1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OF1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OF1 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1rgs|1rgs]], [[1cx4|1cx4]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BCY1, REG1, SRA1, YIL033C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3of1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3of1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3of1 RCSB], [http://www.ebi.ac.uk/pdbsum/3of1 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The major cAMP receptors in eukaryotes are the regulatory (R) subunits of PKA, an allosteric enzyme conserved in fungi through mammals. While mammals have four R-subunit genes, Saccharomyces cerevisiae has only one, Bcy1. To achieve a molecular understanding of PKA activation in yeast and to explore the evolution of cyclic-nucleotide binding (CNB) domains, we solved the structure of cAMP-bound Bcy1(168-416). Surprisingly, the relative orientation of the two CNB domains in Bcy1 is very different from mammalian R-subunits. This quaternary structure is defined primarily by a fungi-specific sequence in the hinge between the alphaB/alphaC helices of the CNB-A domain. The unique interface between the two CNB domains in Bcy1 defines the allosteric mechanism for cooperative activation of PKA by cAMP. Some interface motifs are isoform-specific while others, although conserved, play surprisingly different roles in each R-subunit. Phylogenetic analysis shows that structural differences in Bcy1 are shared by fungi of the subphylum Saccharomycotina.
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==Function==
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Structure of yeast regulatory subunit: a glimpse into the evolution of PKA signaling.,Rinaldi J, Wu J, Yang J, Ralston CY, Sankaran B, Moreno S, Taylor SS Structure. 2010 Nov 10;18(11):1471-82. PMID:21070946<ref>PMID:21070946</ref>
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[[http://www.uniprot.org/uniprot/KAPR_YEAST KAPR_YEAST]] Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA), an effector of the Ras/cAMP pathway. Inhibits PKA activity in the absence of cAMP. cAMP activates PKA and promotes growth and proliferation in response to good nutrient conditions. Together with ZDS1, provides a negative feedback control on the cell wall integrity-signaling pathway by acting as a negative regulator of MAP kinase SLT2/MPK1.<ref>PMID:3037314</ref> <ref>PMID:2823100</ref> <ref>PMID:6243658</ref> <ref>PMID:12704202</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3of1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OF1 OCA].
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</div>
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== References ==
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==See Also==
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<references/>
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*[[CAMP-dependent protein kinase|CAMP-dependent protein kinase]]
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__TOC__
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:021070946</ref><references group="xtra"/><references/>
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Moreno, S.]]
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[[Category: Moreno, S]]
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[[Category: Ralston, C Y.]]
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[[Category: Ralston, C Y]]
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[[Category: Rinaldi, J.]]
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[[Category: Rinaldi, J]]
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[[Category: Sankaran, B.]]
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[[Category: Sankaran, B]]
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[[Category: Taylor, S S.]]
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[[Category: Taylor, S S]]
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[[Category: Wu, J.]]
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[[Category: Wu, J]]
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[[Category: Yang, J.]]
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[[Category: Yang, J]]
[[Category: Camp-dependent protein kinase]]
[[Category: Camp-dependent protein kinase]]
[[Category: Cyclic nucleotide binding domain]]
[[Category: Cyclic nucleotide binding domain]]

Revision as of 06:58, 19 December 2014

Crystal Structure of Bcy1, the Yeast Regulatory Subunit of PKA

3of1, resolution 2.21Å

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