1qv8

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[[Image:1qv8.gif|left|200px]]<br /><applet load="1qv8" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1qv8.gif|left|200px]]
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caption="1qv8, resolution 2.5&Aring;" />
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'''B-DNA Dodecamer d(CGCGAATTCGCG)2 complexed with proamine'''<br />
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{{Structure
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|PDB= 1qv8 |SIZE=350|CAPTION= <scene name='initialview01'>1qv8</scene>, resolution 2.5&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=BBZ:2'-(4-DIMETHYLAMINOPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5'-BI-BENZIMIDAZOLE'>BBZ</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''B-DNA Dodecamer d(CGCGAATTCGCG)2 complexed with proamine'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1QV8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=BBZ:'>BBZ</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QV8 OCA].
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1QV8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QV8 OCA].
==Reference==
==Reference==
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In vitro studies with methylproamine: a potent new radioprotector., Martin RF, Broadhurst S, Reum ME, Squire CJ, Clark GR, Lobachevsky PN, White JM, Clark C, Sy D, Spotheim-Maurizot M, Kelly DP, Cancer Res. 2004 Feb 1;64(3):1067-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14871839 14871839]
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In vitro studies with methylproamine: a potent new radioprotector., Martin RF, Broadhurst S, Reum ME, Squire CJ, Clark GR, Lobachevsky PN, White JM, Clark C, Sy D, Spotheim-Maurizot M, Kelly DP, Cancer Res. 2004 Feb 1;64(3):1067-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14871839 14871839]
[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Clark, G R.]]
[[Category: Clark, G R.]]
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[[Category: minor groove binder]]
[[Category: minor groove binder]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:44:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:43:09 2008''

Revision as of 11:43, 20 March 2008


PDB ID 1qv8

Drag the structure with the mouse to rotate
, resolution 2.5Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



B-DNA Dodecamer d(CGCGAATTCGCG)2 complexed with proamine


Overview

New analogues of the minor groove binding ligand Hoechst 33342 have been investigated in an attempt to improve radioprotective activity. The synthesis, DNA binding, and in vitro radioprotective properties of methylproamine, the most potent derivative, are reported. Experiments with V79 cells have shown that methylproamine is approximately 100-fold more potent than the classical aminothiol radioprotector WR1065. The crystal structures of methylproamine and proamine complexes with the dodecamer d(CGCGAATTCGCG)(2) confirm that the new analogues also are minor groove binders. It is proposed that the DNA-bound methylproamine ligand acts as a reducing agent by an electron transfer mechanism, repairing transient radiation-induced oxidizing species on DNA.

About this Structure

1QV8 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

In vitro studies with methylproamine: a potent new radioprotector., Martin RF, Broadhurst S, Reum ME, Squire CJ, Clark GR, Lobachevsky PN, White JM, Clark C, Sy D, Spotheim-Maurizot M, Kelly DP, Cancer Res. 2004 Feb 1;64(3):1067-70. PMID:14871839

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