3pja

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{{STRUCTURE_3pja| PDB=3pja | SCENE= }}
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==Crystal structure of human C3PO complex==
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===Crystal structure of human C3PO complex===
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<StructureSection load='3pja' size='340' side='right' caption='[[3pja]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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{{ABSTRACT_PUBMED_21552258}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3pja]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PJA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PJA FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Translin, TSN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), Translin associated factor X, TRAX, TSNAX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pja OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pja RCSB], [http://www.ebi.ac.uk/pdbsum/3pja PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Assembly of the RNA-induced silencing complex (RISC) consists of loading duplex (guide-passenger) siRNA onto Argonaute (Ago2) and removing the passenger strand. Ago2 contributes critically to RISC activation by nicking the passenger strand. Here we reconstituted duplex siRNA-initiated RISC activity using recombinant human Ago2 (hAgo2) and C3PO, indicating that C3PO has a critical role in hAgo2-RISC activation. Consistently, genetic depletion of C3PO compromised RNA silencing in mammalian cells. We determined the crystal structure of hC3PO, which reveals an asymmetric octamer barrel consisting of six translin and two TRAX subunits. This asymmetric assembly is critical for the function of C3PO as an endonuclease that cleaves RNA at the interior surface. The current work supports a Dicer-independent mechanism for human RISC activation, in which Ago2 directly binds duplex siRNA and nicks the passenger strand, and then C3PO activates RISC by degrading the Ago2-nicked passenger strand.
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==Function==
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Structure of C3PO and mechanism of human RISC activation.,Ye X, Huang N, Liu Y, Paroo Z, Huerta C, Li P, Chen S, Liu Q, Zhang H Nat Struct Mol Biol. 2011 May 8. PMID:21552258<ref>PMID:21552258</ref>
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[[http://www.uniprot.org/uniprot/TSN_HUMAN TSN_HUMAN]] DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig)/T-cell receptor gene segments. Seems to recognize single-stranded DNA ends generated by staggered breaks occurring at recombination hot spots.<ref>PMID:9111049</ref> <ref>PMID:21552258</ref> Exhibits both single-stranded and double-stranded endoribonuclease activity. May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand.<ref>PMID:9111049</ref> <ref>PMID:21552258</ref> [[http://www.uniprot.org/uniprot/TSNAX_HUMAN TSNAX_HUMAN]] Acts in combination with TSN as an endonuclease involved in the activation of the RNA-induced silencing complex (RISC). Possible role in spermatogenesis.<ref>PMID:12036294</ref> <ref>PMID:21552258</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3pja]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PJA OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:021552258</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Huang, N.]]
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[[Category: Huang, N]]
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[[Category: Zhang, H.]]
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[[Category: Zhang, H]]
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[[Category: 7 alpha helical bundle]]
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[[Category: Alpha helical bundle]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Ribonuclease]]
[[Category: Ribonuclease]]

Revision as of 07:04, 19 December 2014

Crystal structure of human C3PO complex

3pja, resolution 3.00Å

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