1qwj

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[[Image:1qwj.gif|left|200px]]<br /><applet load="1qwj" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1qwj.gif|left|200px]]
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caption="1qwj, resolution 2.8&Aring;" />
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'''The Crystal Structure of Murine CMP-5-N-Acetylneuraminic Acid Synthetase'''<br />
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{{Structure
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|PDB= 1qwj |SIZE=350|CAPTION= <scene name='initialview01'>1qwj</scene>, resolution 2.8&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NCC:CYTIDINE-5'-MONOPHOSPHATE-5-N-ACETYLNEURAMINIC ACID'>NCC</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/N-acylneuraminate_cytidylyltransferase N-acylneuraminate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.43 2.7.7.43]
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|GENE= Cmas ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
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}}
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'''The Crystal Structure of Murine CMP-5-N-Acetylneuraminic Acid Synthetase'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1QWJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=NCC:'>NCC</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/N-acylneuraminate_cytidylyltransferase N-acylneuraminate cytidylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.43 2.7.7.43] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWJ OCA].
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1QWJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWJ OCA].
==Reference==
==Reference==
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The crystal structure of murine CMP-5-N-acetylneuraminic acid synthetase., Krapp S, Munster-Kuhnel AK, Kaiser JT, Huber R, Tiralongo J, Gerardy-Schahn R, Jacob U, J Mol Biol. 2003 Dec 5;334(4):625-37. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14636592 14636592]
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The crystal structure of murine CMP-5-N-acetylneuraminic acid synthetase., Krapp S, Munster-Kuhnel AK, Kaiser JT, Huber R, Tiralongo J, Gerardy-Schahn R, Jacob U, J Mol Biol. 2003 Dec 5;334(4):625-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14636592 14636592]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: N-acylneuraminate cytidylyltransferase]]
[[Category: N-acylneuraminate cytidylyltransferase]]
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[[Category: sugar-activating enzyme]]
[[Category: sugar-activating enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:44:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:43:37 2008''

Revision as of 11:43, 20 March 2008

Image:1qwj.gif


PDB ID 1qwj

Drag the structure with the mouse to rotate
, resolution 2.8Å
Ligands:
Gene: Cmas (Mus musculus)
Activity: N-acylneuraminate cytidylyltransferase, with EC number 2.7.7.43
Coordinates: save as pdb, mmCIF, xml



The Crystal Structure of Murine CMP-5-N-Acetylneuraminic Acid Synthetase


Overview

Sialic acids are activated by CMP-5-N-acetylneuraminic acid synthetase prior to their transfer onto oligo- or polysaccharides. Here, we present the crystal structure of the N-terminal catalytically active domain of the murine 5-N-acetylneuraminic acid synthetase in complex with the reaction product. In contrast to the previously solved structure of 5-N-acetylneuraminic acid synthetase from Neisseria meningitidis and the related CMP-KDO-synthetase of Escherichia coli, the murine enzyme is a tetramer, which was observed with the active sites closed. In this conformation a loop is shifted by 6A towards the active site and thus an essential arginine residue can participate in catalysis. Furthermore, a network of intermolecular salt-bridges and hydrogen bonds in the dimer as well as hydrophobic interfaces between two dimers indicate a cooperative behaviour of the enzyme. In addition, a complex regulation of the enzyme activity is proposed that includes phosphorylation and dephosphorylation.

About this Structure

1QWJ is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

The crystal structure of murine CMP-5-N-acetylneuraminic acid synthetase., Krapp S, Munster-Kuhnel AK, Kaiser JT, Huber R, Tiralongo J, Gerardy-Schahn R, Jacob U, J Mol Biol. 2003 Dec 5;334(4):625-37. PMID:14636592

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