3nt7

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{{STRUCTURE_3nt7| PDB=3nt7 | SCENE= }}
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==Crystal Structure of Vaccinia Virus Uracil DNA Glycosylase R187V Mutant==
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===Crystal Structure of Vaccinia Virus Uracil DNA Glycosylase R187V Mutant===
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<StructureSection load='3nt7' size='340' side='right' caption='[[3nt7]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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{{ABSTRACT_PUBMED_20861259}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3nt7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NT7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NT7 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2owq|2owq]], [[2owr|2owr]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UNG, ACAM3000_MVA_101, MVA101R ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10245 Vaccinia virus])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Uracil-DNA_glycosylase Uracil-DNA glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.27 3.2.2.27] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nt7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nt7 RCSB], [http://www.ebi.ac.uk/pdbsum/3nt7 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Genome replication is inefficient without processivity factors, which tether DNA polymerases to their templates. The vaccinia virus DNA polymerase E9 requires two viral proteins, A20 and D4, for processive DNA synthesis, yet the mechanism of how this tricomplex functions is unknown. This study confirms that these three proteins are necessary and sufficient for processivity, and it focuses on the role of D4, which also functions as a uracil DNA glycosylase (UDG) repair enzyme. A series of D4 mutants was generated to discover which sites are important for processivity. Three point mutants (K126V, K160V, and R187V) which did not function in processive DNA synthesis, though they retained UDG catalytic activity, were identified. The mutants were able to compete with wild-type D4 in processivity assays and retained binding to both A20 and DNA. The crystal structure of R187V was resolved and revealed that the local charge distribution around the substituted residue is altered. However, the mutant protein was shown to have no major structural distortions. This suggests that the positive charges of residues 126, 160, and 187 are required for D4 to function in processive DNA synthesis. Consistent with this is the ability of the conserved mutant K126R to function in processivity. These mutants may help unlock the mechanism by which D4 contributes to processive DNA synthesis.
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==Function==
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Vaccinia virus D4 mutants defective in processive DNA synthesis retain binding to A20 and DNA.,Druck Shudofsky AM, Silverman JE, Chattopadhyay D, Ricciardi RP J Virol. 2010 Dec;84(23):12325-35. Epub 2010 Sep 22. PMID:20861259<ref>PMID:20861259</ref>
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[[http://www.uniprot.org/uniprot/UNG_VACCA UNG_VACCA]] Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. Also part of a heterodimeric processivity factor which potentiates the DNA polymerase activity. Binds to DNA.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3nt7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NT7 OCA].
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</div>
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==Reference==
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==See Also==
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<ref group="xtra">PMID:020861259</ref><references group="xtra"/><references/>
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*[[Uracil-DNA glycosylase|Uracil-DNA glycosylase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Uracil-DNA glycosylase]]
[[Category: Uracil-DNA glycosylase]]
[[Category: Vaccinia virus]]
[[Category: Vaccinia virus]]
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[[Category: Chattopadhyay, D.]]
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[[Category: Chattopadhyay, D]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Uracil-dna glycosylase fold]]
[[Category: Uracil-dna glycosylase fold]]

Revision as of 07:13, 19 December 2014

Crystal Structure of Vaccinia Virus Uracil DNA Glycosylase R187V Mutant

3nt7, resolution 2.40Å

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