This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3olc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{STRUCTURE_3olc| PDB=3olc | SCENE= }}
+
==Crystal structure of the N-terminal region of TopBP1==
-
===Crystal structure of the N-terminal region of TopBP1===
+
<StructureSection load='3olc' size='340' side='right' caption='[[3olc]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-
{{ABSTRACT_PUBMED_20858457}}
+
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[3olc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OLC FirstGlance]. <br>
 +
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 +
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TOPBP1, KIAA0259 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3olc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3olc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3olc RCSB], [http://www.ebi.ac.uk/pdbsum/3olc PDBsum]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Human DNA Topoisomerase IIbeta binding protein 1 (TopBP1) is a modulating protein that plays an essential role in the response to DNA damage. The N-terminal region of TopBP1, which contains predicted BRCA1-carboxy terminal (BRCT) domains 1 and 2, binds to Rad9, a component of the cell cycle checkpoint clamp Rad9-Hus1-Rad1 complex. Here, we report the crystal structure of the TopBP1N-terminal region (residues 1-290) at 2.4A resolution. Interestingly, in addition to the predicted tandem BRCT1-2 repeats (residues 103-284), residues 7-98 form a previously unreported BRCT domain (here, BRCT0). In contrast to both BRCT1 and BRCT2, which possess the conventional phosphopeptide binding residues within a surface pocket, the corresponding pocket in BRCT0 is largely hydrophobic. Structural comparisons together with peptide binding studies indicate that the tandem BRCT1-2 domains are the binding region for phosphorylated Ser387 in Rad9.
-
==Function==
+
Crystal structure of the N-terminal region of human Topoisomerase IIbeta binding protein 1.,Huo YG, Bai L, Xu M, Jiang T Biochem Biophys Res Commun. 2010 Oct 22;401(3):401-5. Epub 2010 Sep 19. PMID:20858457<ref>PMID:20858457</ref>
-
[[http://www.uniprot.org/uniprot/TOPB1_HUMAN TOPB1_HUMAN]] Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR.<ref>PMID:10498869</ref> <ref>PMID:11395493</ref> <ref>PMID:11714696</ref> <ref>PMID:12697828</ref> <ref>PMID:15075294</ref> <ref>PMID:16530042</ref>
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[3olc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OLC OCA].
+
</div>
-
==Reference==
+
==See Also==
-
<ref group="xtra">PMID:020858457</ref><references group="xtra"/><references/>
+
*[[Topoisomerase binding protein|Topoisomerase binding protein]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Bai, L.]]
+
[[Category: Bai, L]]
-
[[Category: Huo, Y G.]]
+
[[Category: Huo, Y G]]
-
[[Category: Jiang, T.]]
+
[[Category: Jiang, T]]
-
[[Category: Xu, M.]]
+
[[Category: Xu, M]]
[[Category: Brct domain]]
[[Category: Brct domain]]
[[Category: Dna binding protein]]
[[Category: Dna binding protein]]
[[Category: Dna repair]]
[[Category: Dna repair]]
[[Category: Rad9]]
[[Category: Rad9]]

Revision as of 07:15, 19 December 2014

Crystal structure of the N-terminal region of TopBP1

3olc, resolution 2.40Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3olc"
Personal tools