3obl
From Proteopedia
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| - | + | ==Crystal structure of the potent anti-HIV cyanobacterial lectin from Oscillatoria Agardhii== | |
| - | + | <StructureSection load='3obl' size='340' side='right' caption='[[3obl]], [[Resolution|resolution]] 1.20Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3obl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Planktothrix_agardhii Planktothrix agardhii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OBL FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CXS:3-CYCLOHEXYL-1-PROPYLSULFONIC+ACID'>CXS</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">OAA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1160 Planktothrix agardhii])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3obl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3obl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3obl RCSB], [http://www.ebi.ac.uk/pdbsum/3obl PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Oscillatoria agardhii agglutinin (OAA) is a recently discovered cyanobacterial lectin that exhibits potent anti-HIV activity. Up to now, only its primary structure and carbohydrate binding data have been available. To elucidate the structural basis for the antiviral mechanism of OAA, we determined the structure of this lectin by x-ray crystallography at 1.2 A resolution and mapped the specific carbohydrate recognition sites of OAA by NMR spectroscopy. The overall architecture of OAA comprises 10 beta-strands that fold into a single, compact, beta-barrel-like domain, creating a unique topology compared with all known protein structures in the Protein Data Bank. OAA sugar binding was tested against Man-9 and various disaccharide components of Man-9. Two symmetric carbohydrate-binding sites were located on the protein, and a preference for Manalpha(1-6)Man-linked sugars was found. Altogether, our structural results explain the antiviral activity OAA and add to the growing body of knowledge about antiviral lectins. | ||
| - | + | Novel fold and carbohydrate specificity of the potent anti-HIV cyanobacterial lectin from Oscillatoria agardhii.,Koharudin LM, Furey W, Gronenborn AM J Biol Chem. 2011 Jan 14;286(2):1588-97. Epub 2010 Oct 19. PMID:20961847<ref>PMID:20961847</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | < | + | </div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Planktothrix agardhii]] | [[Category: Planktothrix agardhii]] | ||
| - | [[Category: Furey, W | + | [[Category: Furey, W]] |
| - | [[Category: Gronenborn, A M | + | [[Category: Gronenborn, A M]] |
| - | [[Category: Koharudin, L M.I | + | [[Category: Koharudin, L M.I]] |
[[Category: Anti-hiv lectin]] | [[Category: Anti-hiv lectin]] | ||
[[Category: High mannose glycan]] | [[Category: High mannose glycan]] | ||
[[Category: Novel beta barrel fold]] | [[Category: Novel beta barrel fold]] | ||
[[Category: Sugar binding protein]] | [[Category: Sugar binding protein]] | ||
Revision as of 07:21, 19 December 2014
Crystal structure of the potent anti-HIV cyanobacterial lectin from Oscillatoria Agardhii
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