1qxm
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1qxm.gif|left|200px]] | + | [[Image:1qxm.gif|left|200px]] |
| - | + | ||
| - | '''Crystal structure of a hemagglutinin component (HA1) from type C Clostridium botulinum''' | + | {{Structure |
| + | |PDB= 1qxm |SIZE=350|CAPTION= <scene name='initialview01'>1qxm</scene>, resolution 1.70Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of a hemagglutinin component (HA1) from type C Clostridium botulinum''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1QXM is a [ | + | 1QXM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum_d_phage Clostridium botulinum d phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXM OCA]. |
==Reference== | ==Reference== | ||
| - | Structural analysis by X-ray crystallography and calorimetry of a haemagglutinin component (HA1) of the progenitor toxin from Clostridium botulinum., Inoue K, Sobhany M, Transue TR, Oguma K, Pedersen LC, Negishi M, Microbiology. 2003 Dec;149(Pt 12):3361-70. PMID:[http:// | + | Structural analysis by X-ray crystallography and calorimetry of a haemagglutinin component (HA1) of the progenitor toxin from Clostridium botulinum., Inoue K, Sobhany M, Transue TR, Oguma K, Pedersen LC, Negishi M, Microbiology. 2003 Dec;149(Pt 12):3361-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14663070 14663070] |
[[Category: Clostridium botulinum d phage]] | [[Category: Clostridium botulinum d phage]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 25: | Line 34: | ||
[[Category: trefoil]] | [[Category: trefoil]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:44:08 2008'' |
Revision as of 11:44, 20 March 2008
| |||||||
| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a hemagglutinin component (HA1) from type C Clostridium botulinum
Overview
Botulism food poisoning is caused primarily by ingestion of the Clostridium botulinum neurotoxin (BoNT). The 1300 amino acid BoNT forms a progenitor toxin (PTX) that, when associated with a number of other proteins, increases its oral toxicity by protecting it from the low pH of the stomach and from intestinal proteases. One of these associated proteins, HA1, has also been suggested to be involved with internalization of the toxin into the bloodstream by binding to oligosaccharides lining the intestine. Here is reported the crystal structure of HA1 from type C Clostridium botulinum at a resolution of 1.7 Angstrom. The protein consists of two beta-trefoil domains and bears structural similarities to the lectin B-chain from the deadly plant toxin ricin. Based on structural comparison to the ricin B-chain lactose-binding sites, residues of type A HA1 were selected and mutated. The D263A and N285A mutants lost the ability to bind carbohydrates containing galactose moieties, implicating these residues in carbohydrate binding.
About this Structure
1QXM is a Single protein structure of sequence from Clostridium botulinum d phage. Full crystallographic information is available from OCA.
Reference
Structural analysis by X-ray crystallography and calorimetry of a haemagglutinin component (HA1) of the progenitor toxin from Clostridium botulinum., Inoue K, Sobhany M, Transue TR, Oguma K, Pedersen LC, Negishi M, Microbiology. 2003 Dec;149(Pt 12):3361-70. PMID:14663070
Page seeded by OCA on Thu Mar 20 13:44:08 2008
