3qio
From Proteopedia
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| - | + | ==Crystal Structure of HIV-1 RNase H with engineered E. coli loop and N-hydroxy quinazolinedione inhibitor== | |
| - | + | <StructureSection load='3qio' size='340' side='right' caption='[[3qio]], [[Resolution|resolution]] 1.40Å' scene=''> | |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3qio]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Hiv-1_m:b_hxb2r Hiv-1 m:b_hxb2r]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QIO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QIO FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=QID:3-HYDROXY-6-(PHENYLSULFONYL)QUINAZOLINE-2,4(1H,3H)-DIONE'>QID</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hyf|3hyf]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gag-pol ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11706 HIV-1 M:B_HXB2R])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qio OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qio RCSB], [http://www.ebi.ac.uk/pdbsum/3qio PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Human immunodeficiency virus (HIV-1) RNase H breaks down the intermediate RNA/DNA hybrids during reverse transcription, requiring two divalent metal ions for activity. Pyrimidinol carboxylic acid and N-hydroxy quinazolinedione inhibitors were designed to coordinate the two metal ions in the active site of RNase H. High resolution (1.4A-2.1A) crystal structures were determined with isolated RNase H domain and RT which permit accurate assessment of the metal and water environment at the active site. The geometry of the metal coordination suggests that the inhibitors mimic a substrate state prior to phosphodiester catalysis. Surface plasmon resonance studies confirm metal-dependent binding to RNase H and demonstrate that the inhibitors do not bind at the polymerase active site of RT. Additional evaluation of the RNase H site reveals an open protein surface with few additional interactions to optimize active site inhibitors. | ||
| - | + | Structural and Binding Analysis of Pyrimidinol Carboxylic Acid and N-hydroxy Quinazolinedione HIV-1 RNase H Inhibitors.,Lansdon EB, Liu Q, Leavitt SA, Balakrishnan M, Perry JK, Lancaster-Moyer C, Kutty N, Liu X, Squires NH, Watkins WJ, Kirschberg TA Antimicrob Agents Chemother. 2011 Apr 4. PMID:21464257<ref>PMID:21464257</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Hiv-1 m:b_hxb2r]] | [[Category: Hiv-1 m:b_hxb2r]] | ||
| - | [[Category: Lansdon, E B | + | [[Category: Lansdon, E B]] |
| - | [[Category: Liu, Q | + | [[Category: Liu, Q]] |
[[Category: Hiv-1]] | [[Category: Hiv-1]] | ||
[[Category: Hydrolase-inhibitor complex]] | [[Category: Hydrolase-inhibitor complex]] | ||
Revision as of 10:15, 19 December 2014
Crystal Structure of HIV-1 RNase H with engineered E. coli loop and N-hydroxy quinazolinedione inhibitor
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