1r19
From Proteopedia
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| - | [[Image:1r19.jpg|left|200px]] | + | [[Image:1r19.jpg|left|200px]] |
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| - | '''Crystal Structure Analysis of S.epidermidis adhesin SdrG binding to Fibrinogen (Apo structure)''' | + | {{Structure |
| + | |PDB= 1r19 |SIZE=350|CAPTION= <scene name='initialview01'>1r19</scene>, resolution 3.51Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= sdrG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1282 Staphylococcus epidermidis]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure Analysis of S.epidermidis adhesin SdrG binding to Fibrinogen (Apo structure)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1R19 is a [ | + | 1R19 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R19 OCA]. |
==Reference== | ==Reference== | ||
| - | A "dock, lock, and latch" structural model for a staphylococcal adhesin binding to fibrinogen., Ponnuraj K, Bowden MG, Davis S, Gurusiddappa S, Moore D, Choe D, Xu Y, Hook M, Narayana SV, Cell. 2003 Oct 17;115(2):217-28. PMID:[http:// | + | A "dock, lock, and latch" structural model for a staphylococcal adhesin binding to fibrinogen., Ponnuraj K, Bowden MG, Davis S, Gurusiddappa S, Moore D, Choe D, Xu Y, Hook M, Narayana SV, Cell. 2003 Oct 17;115(2):217-28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14567919 14567919] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus epidermidis]] | [[Category: Staphylococcus epidermidis]] | ||
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[[Category: sdrg native]] | [[Category: sdrg native]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:45:30 2008'' |
Revision as of 11:45, 20 March 2008
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| , resolution 3.51Å | |||||||
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| Gene: | sdrG (Staphylococcus epidermidis) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure Analysis of S.epidermidis adhesin SdrG binding to Fibrinogen (Apo structure)
Overview
Gram-positive pathogens such as staphylococci contain multiple cell wall-anchored proteins that serve as an interface between the microbe and its environment. Some of these proteins act as adhesins and mediate bacterial attachment to host tissues. SdrG is a cell wall-anchored adhesin from Staphylococcus epidermidis that binds to the Bbeta chain of human fibrinogen (Fg) and is necessary and sufficient for bacterial attachment to Fg-coated biomaterials. Here, we present the crystal structures of the ligand binding region of SdrG as an apoprotein and in complex with a synthetic peptide analogous to its binding site in Fg. Analysis of the crystal structures, along with mutational studies of both the protein and of the peptide, reveals that SdrG binds to its ligand with a dynamic "dock, lock, and latch" mechanism. We propose that this mechanism represents a general mode of ligand binding for structurally related cell wall-anchored proteins of gram-positive bacteria.
About this Structure
1R19 is a Single protein structure of sequence from Staphylococcus epidermidis. Full crystallographic information is available from OCA.
Reference
A "dock, lock, and latch" structural model for a staphylococcal adhesin binding to fibrinogen., Ponnuraj K, Bowden MG, Davis S, Gurusiddappa S, Moore D, Choe D, Xu Y, Hook M, Narayana SV, Cell. 2003 Oct 17;115(2):217-28. PMID:14567919
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