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3r3m
From Proteopedia
(Difference between revisions)
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| - | + | ==Crystal structure of the FAF1 UBX domain== | |
| - | === | + | <StructureSection load='3r3m' size='340' side='right' caption='[[3r3m]], [[Resolution|resolution]] 3.00Å' scene=''> |
| - | + | == Structural highlights == | |
| + | <table><tr><td colspan='2'>[[3r3m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R3M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R3M FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FAF1, UBXD12, UBXN3A, CGI-03 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r3m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r3m RCSB], [http://www.ebi.ac.uk/pdbsum/3r3m PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The hexameric AAA ATPase p97 is involved in several human proteinopathies and mediates ubiquitin-dependent protein degradation among other essential cellular processes. Via its N-terminal domain (N domain), p97 interacts with multiple regulatory cofactors including the UFD1/NPL4 heterodimer and members of the "ubiquitin regulatory X" (UBX) domain protein family; however, the principles governing cofactor selectivity remain to be deciphered. Our crystal structure of the FAS-associated factor 1 (FAF1)UBX domain in complex with the p97N domain reveals that the signature Phe-Pro-Arg motif known to be crucial for interactions of UBX domains with p97 adopts a cis-proline configuration, in contrast to a cis-trans mixture we derive for the isolated FAF1UBX domain. Biochemical studies confirm that binding critically depends on a proline at this position. Furthermore, we observe that the UBX proteins FAF1 and UBXD7 only bind to p97-UFD1/NPL4, but not free p97, thus demonstrating for the first time a hierarchy in p97-cofactor interactions. | ||
| - | + | Hierarchical Binding of Cofactors to the AAA ATPase p97.,Hanzelmann P, Buchberger A, Schindelin H Structure. 2011 Jun 8;19(6):833-43. PMID:21645854<ref>PMID:21645854</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Haenzelmann, P | + | [[Category: Haenzelmann, P]] |
| - | [[Category: Schindelin, H | + | [[Category: Schindelin, H]] |
[[Category: Apoptosis]] | [[Category: Apoptosis]] | ||
[[Category: Beta grasp fold]] | [[Category: Beta grasp fold]] | ||
[[Category: P97]] | [[Category: P97]] | ||
[[Category: Phosphorylation]] | [[Category: Phosphorylation]] | ||
Revision as of 10:39, 19 December 2014
Crystal structure of the FAF1 UBX domain
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