1r27
From Proteopedia
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| - | [[Image:1r27.gif|left|200px]] | + | [[Image:1r27.gif|left|200px]] |
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| - | '''Crystal Structure of NarGH complex''' | + | {{Structure |
| + | |PDB= 1r27 |SIZE=350|CAPTION= <scene name='initialview01'>1r27</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MO:MOLYBDENUM+ATOM'>MO</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene> and <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE GUANOSINE DINUCLEOTIDE'>MGD</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Nitrate_reductase Nitrate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.4 1.7.99.4] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of NarGH complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1R27 is a [ | + | 1R27 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R27 OCA]. |
==Reference== | ==Reference== | ||
| - | Architecture of NarGH reveals a structural classification of Mo-bisMGD enzymes., Jormakka M, Richardson D, Byrne B, Iwata S, Structure. 2004 Jan;12(1):95-104. PMID:[http:// | + | Architecture of NarGH reveals a structural classification of Mo-bisMGD enzymes., Jormakka M, Richardson D, Byrne B, Iwata S, Structure. 2004 Jan;12(1):95-104. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14725769 14725769] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Nitrate reductase]] | [[Category: Nitrate reductase]] | ||
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[[Category: beta barrel; x-ray crystallography]] | [[Category: beta barrel; x-ray crystallography]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:45:53 2008'' |
Revision as of 11:45, 20 March 2008
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| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Activity: | Nitrate reductase, with EC number 1.7.99.4 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of NarGH complex
Overview
The structure of the catalytic and electron-transfer subunits (NarGH) of the integral membrane protein, respiratory nitrate reductase (Nar) has been determined to 2.0 A resolution revealing the molecular architecture of this Mo-bisMGD (molybdopterin-guanine-dinucleotide) containing enzyme which includes a previously undetected FeS cluster. Nar, together with the related enzyme formate dehydrogenase (Fdh-N), is a key enzyme in the generation of proton motive force across the membrane in Escherichia coli nitrate respiration. A comparative study revealed that Nar and Fdh-N employ different approaches for acquiring substrate, reflecting different catalytic mechanisms. Nar uses a very narrow and nonpolar substrate-conducting cavity with a nonspecific substrate binding site, whereas Fdh-N accommodates a wider, positively charged substrate-conducting cavity with a more specific substrate binding site. The Nar structure also demonstrates the first example of an Asp side chain acting as a Mo ligand providing a structural basis for the classification of Mo-bisMGD enzymes.
About this Structure
1R27 is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Architecture of NarGH reveals a structural classification of Mo-bisMGD enzymes., Jormakka M, Richardson D, Byrne B, Iwata S, Structure. 2004 Jan;12(1):95-104. PMID:14725769
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