3qzv

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{{STRUCTURE_3qzv| PDB=3qzv | SCENE= }}
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==Crystal Structure of BPTF PHD-linker-bromo in complex with histone H4K12ac peptide==
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===Crystal Structure of BPTF PHD-linker-bromo in complex with histone H4K12ac peptide===
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<StructureSection load='3qzv' size='340' side='right' caption='[[3qzv]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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{{ABSTRACT_PUBMED_21596426}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3qzv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QZV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QZV FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qzs|3qzs]], [[3qzt|3qzt]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BPTF, FAC1, FALZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qzv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qzv RCSB], [http://www.ebi.ac.uk/pdbsum/3qzv PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Little is known about how combinations of histone marks are interpreted at the level of nucleosomes. The second PHD finger of human BPTF is known to specifically recognize histone H3 when methylated on lysine 4 (H3K4me2/3). Here, we examine how additional heterotypic modifications influence BPTF binding. Using peptide surrogates, three acetyllysine ligands are indentified for a PHD-adjacent bromodomain in BPTF via systematic screening and biophysical characterization. Although the bromodomain displays limited discrimination among the three possible acetyllysines at the peptide level, marked selectivity is observed for only one of these sites, H4K16ac, in combination with H3K4me3 at the mononucleosome level. In support, these two histone marks constitute a unique trans-histone modification pattern that unambiguously resides within a single nucleosomal unit in human cells, and this module colocalizes with these marks in the genome. Together, our data call attention to nucleosomal patterning of covalent marks in dictating critical chromatin associations.
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==Function==
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Recognition of a Mononucleosomal Histone Modification Pattern by BPTF via Multivalent Interactions.,Ruthenburg AJ, Li H, Milne TA, Dewell S, McGinty RK, Yuen M, Ueberheide B, Dou Y, Muir TW, Patel DJ, Allis CD Cell. 2011 May 27;145(5):692-706. Epub 2011 May 19. PMID:21596426<ref>PMID:21596426</ref>
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[[http://www.uniprot.org/uniprot/BPTF_HUMAN BPTF_HUMAN]] Histone-binding component of NURF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. Specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. May also regulate transcription through direct binding to DNA or transcription factors.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3qzv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QZV OCA].
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</div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:021596426</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Li, H.]]
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[[Category: Li, H]]
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[[Category: Patel, D J.]]
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[[Category: Patel, D J]]
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[[Category: Ruthenburg, A J.]]
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[[Category: Ruthenburg, A J]]
[[Category: Alpha helix bundle]]
[[Category: Alpha helix bundle]]
[[Category: Histone h4]]
[[Category: Histone h4]]

Revision as of 10:47, 19 December 2014

Crystal Structure of BPTF PHD-linker-bromo in complex with histone H4K12ac peptide

3qzv, resolution 2.00Å

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