3r04

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{{STRUCTURE_3r04| PDB=3r04 | SCENE= }}
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==The discovery of novel benzofuran-2-carboxylic acids as potent Pim-1 inhibitors==
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===The discovery of novel benzofuran-2-carboxylic acids as potent Pim-1 inhibitors===
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<StructureSection load='3r04' size='340' side='right' caption='[[3r04]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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{{ABSTRACT_PUBMED_21507633}}
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3r04]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R04 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R04 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=UNQ:5-{6-[(TRANS-4-AMINOCYCLOHEXYL)AMINO]PYRAZIN-2-YL}-1-BENZOFURAN-2-CARBOXYLIC+ACID'>UNQ</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3r00|3r00]], [[3r01|3r01]], [[3r02|3r02]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PIM1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r04 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r04 RCSB], [http://www.ebi.ac.uk/pdbsum/3r04 PDBsum]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Novel benzofuran-2-carboxylic acids, exemplified by 29, 38 and 39, have been discovered as potent Pim-1 inhibitors using fragment based screening followed by X-ray structure guided medicinal chemistry optimization. The compounds demonstrate potent inhibition against Pim-1 and Pim-2 in enzyme assays. Compound 29 has been tested in the Ambit 442 kinase panel and demonstrates good selectivity for the Pim kinase family. X-ray structures of the inhibitor/Pim-1 binding complex reveal important salt-bridge and hydrogen bond interactions mediated by the compound's carboxylic acid and amino groups.
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==Function==
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The discovery of novel benzofuran-2-carboxylic acids as potent Pim-1 inhibitors.,Xiang Y, Hirth B, Asmussen G, Biemann HP, Bishop KA, Good A, Fitzgerald M, Gladysheva T, Jain A, Jancsics K, Liu J, Metz M, Papoulis A, Skerlj R, Stepp JD, Wei RR Bioorg Med Chem Lett. 2011 May 15;21(10):3050-6. Epub 2011 Mar 16. PMID:21507633<ref>PMID:21507633</ref>
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[[http://www.uniprot.org/uniprot/PIM1_HUMAN PIM1_HUMAN]] Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, an other proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B,induces 14-3-3-proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis.<ref>PMID:1825810</ref> <ref>PMID:10664448</ref> <ref>PMID:12431783</ref> <ref>PMID:15528381</ref> <ref>PMID:16356754</ref> <ref>PMID:18593906</ref> <ref>PMID:19749799</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[3r04]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R04 OCA].
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</div>
==See Also==
==See Also==
*[[Proto-oncogene serine/threonine-protein kinase|Proto-oncogene serine/threonine-protein kinase]]
*[[Proto-oncogene serine/threonine-protein kinase|Proto-oncogene serine/threonine-protein kinase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:021507633</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Non-specific serine/threonine protein kinase]]
[[Category: Non-specific serine/threonine protein kinase]]
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[[Category: Asmussen, G.]]
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[[Category: Asmussen, G]]
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[[Category: Biemann, H P.]]
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[[Category: Biemann, H P]]
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[[Category: Fitzgerald, M.]]
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[[Category: Fitzgerald, M]]
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[[Category: Gladysheva, T.]]
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[[Category: Gladysheva, T]]
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[[Category: Good, A.]]
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[[Category: Good, A]]
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[[Category: Hirth, B.]]
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[[Category: Hirth, B]]
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[[Category: Jancsics, K.]]
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[[Category: Jancsics, K]]
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[[Category: Liu, J.]]
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[[Category: Liu, J]]
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[[Category: Metz, M.]]
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[[Category: Metz, M]]
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[[Category: Papoulis, A.]]
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[[Category: Papoulis, A]]
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[[Category: Skerlj, R.]]
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[[Category: Skerlj, R]]
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[[Category: Stepp, D J.]]
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[[Category: Stepp, D J]]
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[[Category: Wei, R R.]]
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[[Category: Wei, R R]]
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[[Category: Xiang, Y.]]
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[[Category: Xiang, Y]]
[[Category: Inhibitor]]
[[Category: Inhibitor]]
[[Category: Kinae]]
[[Category: Kinae]]
[[Category: Pim1]]
[[Category: Pim1]]
[[Category: Transferase-transferase inhibitor complex]]
[[Category: Transferase-transferase inhibitor complex]]

Revision as of 10:48, 19 December 2014

The discovery of novel benzofuran-2-carboxylic acids as potent Pim-1 inhibitors

3r04, resolution 1.70Å

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