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1r3g
From Proteopedia
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| - | [[Image:1r3g.gif|left|200px]] | + | [[Image:1r3g.gif|left|200px]] |
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| - | '''1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues''' | + | {{Structure |
| + | |PDB= 1r3g |SIZE=350|CAPTION= <scene name='initialview01'>1r3g</scene>, resolution 1.16Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1R3G is a [ | + | 1R3G is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R3G OCA]. |
==Reference== | ==Reference== | ||
| - | 2'-O-[2-(guanidinium)ethyl]-modified oligonucleotides: stabilizing effect on duplex and triplex structures., Prakash TP, Puschl A, Lesnik E, Mohan V, Tereshko V, Egli M, Manoharan M, Org Lett. 2004 Jun 10;6(12):1971-4. PMID:[http:// | + | 2'-O-[2-(guanidinium)ethyl]-modified oligonucleotides: stabilizing effect on duplex and triplex structures., Prakash TP, Puschl A, Lesnik E, Mohan V, Tereshko V, Egli M, Manoharan M, Org Lett. 2004 Jun 10;6(12):1971-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15176796 15176796] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Egli, M.]] | [[Category: Egli, M.]] | ||
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[[Category: dna]] | [[Category: dna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:46:19 2008'' |
Revision as of 11:46, 20 March 2008
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| , resolution 1.16Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues
Overview
[structure: see text] Oligonucleotides with a novel 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modification have been synthesized using a novel protecting group strategy for the guanidinium group. This modification enhances the binding affinity of oligonucleotides to RNA as well as duplex DNA (DeltaT(m) 3.2 degrees C per modification). The 2'-O-GE modified oligonucleotides exhibited exceptional resistance to nuclease degradation. The crystal structure of a palindromic duplex formed by a DNA oligonucleotide with a single 2'-O-GE modification was solved at 1.16 A resolution.
About this Structure
1R3G is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
2'-O-[2-(guanidinium)ethyl]-modified oligonucleotides: stabilizing effect on duplex and triplex structures., Prakash TP, Puschl A, Lesnik E, Mohan V, Tereshko V, Egli M, Manoharan M, Org Lett. 2004 Jun 10;6(12):1971-4. PMID:15176796
Page seeded by OCA on Thu Mar 20 13:46:19 2008
Categories: Protein complex | Egli, M. | Lesnik, E. | Manoharan, M. | Prakash, T P. | Puschl, A. | Tereshko, V. | MG | Dna
