1r4g

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[[Image:1r4g.jpg|left|200px]]<br /><applet load="1r4g" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1r4g.jpg|left|200px]]
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caption="1r4g" />
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'''Solution structure of the Sendai virus protein X C-subdomain'''<br />
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{{Structure
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|PDB= 1r4g |SIZE=350|CAPTION= <scene name='initialview01'>1r4g</scene>
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|SITE=
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|LIGAND=
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|ACTIVITY= [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48]
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|GENE= P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11191 Sendai virus])
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}}
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'''Solution structure of the Sendai virus protein X C-subdomain'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1R4G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sendai_virus Sendai virus]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R4G OCA].
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1R4G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sendai_virus Sendai virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R4G OCA].
==Reference==
==Reference==
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Structure and dynamics of the nucleocapsid-binding domain of the Sendai virus phosphoprotein in solution., Blanchard L, Tarbouriech N, Blackledge M, Timmins P, Burmeister WP, Ruigrok RW, Marion D, Virology. 2004 Feb 20;319(2):201-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14980481 14980481]
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Structure and dynamics of the nucleocapsid-binding domain of the Sendai virus phosphoprotein in solution., Blanchard L, Tarbouriech N, Blackledge M, Timmins P, Burmeister WP, Ruigrok RW, Marion D, Virology. 2004 Feb 20;319(2):201-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14980481 14980481]
[[Category: RNA-directed RNA polymerase]]
[[Category: RNA-directed RNA polymerase]]
[[Category: Sendai virus]]
[[Category: Sendai virus]]
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[[Category: three helix-bundle]]
[[Category: three helix-bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:47:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:46:42 2008''

Revision as of 11:46, 20 March 2008


PDB ID 1r4g

Drag the structure with the mouse to rotate
Gene: P (Sendai virus)
Activity: RNA-directed RNA polymerase, with EC number 2.7.7.48
Coordinates: save as pdb, mmCIF, xml



Solution structure of the Sendai virus protein X C-subdomain


Overview

The RNA-dependent RNA polymerase of the Sendai virus (SeV) consists of the large protein (L) and the phosphoprotein (P). P plays a crucial role in the enzyme by positioning L (which carries the polymerase activity) onto the matrix for transcription and replication formed by the RNA and the nucleoprotein, the N-RNA. P has a modular structure with distinct functional domains: an N-terminal domain involved in binding to N degrees (N that is not yet bound to RNA) and a C-terminal domain that carries the oligomerisation domain, the N-RNA binding domain and the L binding domain and that, combined with L, is active in transcription. Structural data have previously been obtained on the N-terminal domain and on the oligomerisation domain of P, but not yet on its N-RNA binding domain (also-called the X protein). Here we present an NMR and a small angle neutron scattering study of the SeV X protein. We show that this molecule presents two subdomains linked by an 11-residue linker, with the N-subdomain lacking a well-defined conformation. The 3D structure of the C-subdomain consists of three alpha-helices revealing an asymmetric charge distribution that may be important for binding to RNA-bound nucleoprotein. The structure of the entire C-terminal domain of P is modelled from its constituent parts in combination with small angle scattering data on this domain.

About this Structure

1R4G is a Single protein structure of sequence from Sendai virus. Full crystallographic information is available from OCA.

Reference

Structure and dynamics of the nucleocapsid-binding domain of the Sendai virus phosphoprotein in solution., Blanchard L, Tarbouriech N, Blackledge M, Timmins P, Burmeister WP, Ruigrok RW, Marion D, Virology. 2004 Feb 20;319(2):201-11. PMID:14980481

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